We narrowed to 9,975 results for: Uty

1. iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP

   Plasmid

   #204723

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKL11-GCaMP6f bb100

   Plasmid

   #158983

   Purpose

   Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   GCaMP6f
   

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#100833

   Promoter

   Bba_J23100 (sigma 70)

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSG86-J23151-LasR

   Plasmid

   #102456

   Purpose

   Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   LasR (lasR )
   

   Expression

   Bacterial

   Promoter

   J23151

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBlueKan+cysMPro

   Plasmid

   #187879

   Purpose

   Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

   Depositor

   Gaylen Uhlich

   Article

   Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   CysM

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL4164 (pEffector_IscB,ωRNA)

   Plasmid

   #210874

   Purpose

   Expresses GstIscB and native ωRNA from constitutive promoters

   Depositor

   Samuel H. Sternberg

   Article

   Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

   Insert

   GstIscB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD132

   Plasmid

   #119885

   Purpose

   Integration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   BbaJ23104_EYFP
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExp_CAG:EBFP2

   Plasmid

   #51790

   Purpose

   Constitutively expresses blue fluorescent protein, EBFP2

   Depositor

   Ron Weiss

   Article

   Davidsohn et al ACS Synth Biol. 2014 Nov 17.

   Insert

   EBFP2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTORGm43GWY

   Plasmid

   #112906

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

   Type

   Empty backbone

   Use

   Oomycete expression

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB1C3-J23110-B0034-mRFP1_Violet

   Plasmid

   #160447

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

   Insert

   promoter, RBS, mRFP1E_Violet
   

   Use

   Synthetic Biology

   Mutation

   BioBrick sites removed

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pExchange_tdk_BT1311p-tetR-2

    Plasmid

    #90326

    Purpose

    Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Insert

    TetR
    

    Expression

    Bacterial

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p006-GFP-weak

    Plasmid

    #108314

    Purpose

    GFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Expression

    Bacterial

    Promoter

    pFAB4282

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe puro IkB alpha M

    Plasmid

    #12332

    Depositor

    Inder Verma

    Insert

    IkB alpha M (Nfkbia Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Dominant-negative mutant. Many signal transductio…

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pSG28-J23151-AraC

    Plasmid

    #102453

    Purpose

    Expresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    AraC (araC )
    

    Expression

    Bacterial

    Promoter

    J23151

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)

    Plasmid

    #92402

    Purpose

    Constitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

    Depositor

    Periklis Pantazis

    Article

    Kalyviotis et al (2020) Behavioral and Neural Genetics of

    Inserts

    Dendra2
    Cerulean
    

    Use

    Zebrafish expression

    Tags

    H2B tag and No tag, cytosolic expression

    Promoter

    bactin2

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB1C3-J23110-B0034-mRFP1E

    Plasmid

    #160442

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1
    

    Use

    Synthetic Biology

    Mutation

    BioBrick sites removed

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT-DestFLAGHA_HuB

    Plasmid

    #65758

    Purpose

    constitutively expresses FLAG-HA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pSL4369 (pEffector_TnpB2,ωRNA)

    Plasmid

    #210871

    Purpose

    Expresses GstTnpB2 and native ωRNA from constitutive promoters

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    GstTnpB2
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJYDN3

    Plasmid

    #162456

    Purpose

    Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Type

    Empty backbone

    Use

    Shuttle vector bacteria/yeast

    Tags

    ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-Cut-P(11)4

    Plasmid

    #185785

    Purpose

    Encodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    Cut-P(11)4
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCOLA-T7-WspR:D70E

    Plasmid

    #79164

    Purpose

    Expresses constitutively active WspR D70E under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    WspR (wspR P. areuginosa)
    

    Expression

    Bacterial

    Mutation

    D70E

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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