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pGas-miRFP670nano

Plasmid

#127441

Purpose

G protein alpha-s internally tagged with miRFP670nano

Depositor

Vladislav Verkhusha

Publication

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G protein alpha-s-miRFP670nano

Expression

Mammalian

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-1 [N97A/31R]

Plasmid

#114554

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-1 (Rat). Derived from hybridoma N97A/31.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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LZF40: hSyn-Cre

Plasmid

#104124

Purpose

synOptopatch construct

Depositor

Adam Cohen

Publication

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

Cre

Use

Lentiviral

Expression

Mammalian

Promoter

human synapsin I

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-3 [N110/29.4R]

Plasmid

#114518

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-3 (Rat). Derived from hybridoma N110/29.4.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-HaloTag-WPRE

Plasmid

#104108

Purpose

Expresses HaloTag under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Publication

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HaloTag

Expression

Mammalian

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pROS12

Plasmid

#107926

Purpose

hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmiRFP-Vimentin-17-C

Plasmid

#108414

Purpose

Vimentin fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Vimentin (VIM Human)

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pOD2045-intControl

Plasmid

#100005

Purpose

MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).

Depositor

Karen Oegema

Publication

Wang et al Development. 2017 Jun 15. pii: dev.15009

Inserts

zif-1 (zif-1 Nematode)
unc-119

Use

Targeting vector for mos1 transposon mediated sin…

Available since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-yki:V5-S111A, S168A, S250A

Plasmid

#85620

Purpose

Expresses V5 tagged triple mutant yki in Drosophila.

Depositor

Kenneth Irvine

Publication

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S111A,S168,S250A

Tags

V5

Expression

Insect

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA TdR

Plasmid

#80940

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.

Depositor

George Church

Publication

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-CmCHS-MsCHI

Plasmid

#73394

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Publication

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mTagBFP2-A_UTR

Plasmid

#68479

Purpose

Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mTagBFP2-A_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "A" added to 3&#x…

Promoter

CMV and pGK

Available since

Nov. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCmR2

Plasmid

#59015

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Publication

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
GFP KNL1 wt hardened and RVSF->AAAA

Plasmid

#45225

Depositor

Iain Cheeseman, Michael Lampson

Publication

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

GFP KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)

Mutation

RVSF mutated to AAAA

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
pCMV_NESPylRS(WT)_hU6tRNAPyl

Plasmid

#223509

Purpose

PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

Depositor

Edward Lemke

Publication

Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

Inserts

NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
tRNA Pyl

Expression

Mammalian

Promoter

CMV, SP6 and U6

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL685

Plasmid

#205592

Purpose

NEPE for prime editing in rice

Depositor

Yong Zhang

Publication

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 NES visual barcode

Plasmid

#158667

Purpose

tagging clonal populations with NES mTAGBFP2 fluorescent protein. Adding NES to mTAGBFP2 WC

Depositor

Ravid Straussman

Publication

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE651

Plasmid

#153229

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pemiRFP703-myosin

Plasmid

#136565

Purpose

LC-Myosin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

emiRFP703-myosin

Tags

emiRFP703

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-SAP97 [K64/15R]

Plasmid

#128624

Purpose

Mammalian Expression Plasmid of anti-SAP97 (Rat). Derived from hybridoma K64/15.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SAP97 (Rat) recombinant mouse monoclonal antibody (Dlg1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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