We narrowed to 7,199 results for: RAP

1. FKBP-CD28TMD-NTEVp(WT)

   Plasmid

   #137826

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(WT)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pediculus humanus corporis PINK1 (143 - 575)

   Plasmid

   #110751

   Purpose

   Bacterial expression of PhPINK1 (crystallography)

   Depositor

   David Komander

   Article

   Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

   Insert

   Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
   

   Use

   Tags

   N-His6-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPBpuro-LDR

   Plasmid

   #173857

   Purpose

   Encoding a part of the rapamycin-induced dimerization system.

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   a Lyn11-targeted FRB (LDR)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPTXLUC

   Plasmid

   #68504

   Purpose

   cyclic nucleotide reporter for bacteria or plant cells

   Depositor

   Helen Irving

   Article

   Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

   Insert

   OPTX promoter (AT1G33440 Mustard Weed)
   

   Use

   Tags

   luciferase

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   OPTX

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-P4:gs:nls:FKBP

   Plasmid

   #135984

   Purpose

   Expresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the FKBP domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CP1300-CBEmax

   Plasmid

   #119805

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   rApobec1-nSpCas9 (CP1300) -UGI-UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGH51

   Plasmid

   #107255

   Purpose

   HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.

   Depositor

   Erin O'Shea

   Article

   Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

   Insert

   HU-FRB
   

   Use

   Synthetic Biology

   Tags

   FRB

   Expression

   Mutation

   Promoter

   native HU promoter

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-eBE-S1

   Plasmid

   #101092

   Purpose

   Expresses eBE-S1 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

   Insert

   eBE-S1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1alphaFEM1AalphaFLAG

   Plasmid

   #236460

   Purpose

   transient overexpression of FEM1A in mammalian cells

   Depositor

   Michele Pagano

   Article

   Dankert et al Cell Cycle. 2017 Mar 19;16(6):556-564. d

   Insert

   FEM1A (FEM1A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. MZB139

    Plasmid

    #217838

    Purpose

    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    SMT3 (SMT3 Budding Yeast)
    

    Use

    Tags

    10x His and SUMO (SMT3)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. MZB164

    Plasmid

    #217842

    Purpose

    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    HaloTag
    

    Use

    Tags

    10x His, HaloTag, and SUMO (SMT3)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD44I-c011

    Plasmid

    #175449

    Purpose

    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FKBP-EGFP-PML peptide

    Plasmid

    #175247

    Purpose

    Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    PML (PML Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFOSGE_CUS1B05

    Plasmid

    #71077

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    MED20 (MED20 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCMV R-CEPIA1er

    Plasmid

    #58216

    Purpose

    Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    R-CEPIA1er
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-alphasynuclein-A53T

    Plasmid

    #40823

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A53T (aggregates more rapidly than the wild-type …

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV G-CEPIA1er

    Plasmid

    #58215

    Purpose

    Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    G-CEPIA1er
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. FRB_nSuMMVp

    Plasmid

    #118970

    Purpose

    Expresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nSuMMVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

    Plasmid

    #32702

    Purpose

    Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Insert

    eGFP
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3XFlagNICD1

    Plasmid

    #20183

    Depositor

    Raphael Kopan

    Article

    Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

    Insert

    Notch 1 Intracellular Domain (Notch1 Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Contains murine Notch 1 Intracellular Domain (Val…

    Promoter

    CMV

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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