We narrowed to 18,247 results for: multi
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Plasmid#115662PurposeExpresses AcrVA1 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA1
UseLentiviralExpressionMammalianPromoterEF-1a promoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cdk9-Halo donor
Plasmid#113113PurposeDonor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCdk9 donor region including homology arms and HaloTag
UseSynthetic BiologyAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-SNAPf-WPRE
Plasmid#104106PurposeExpresses SNAPf under the TRE promoter in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSNAPf
ExpressionMammalianPromoterTREAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pETDuet RagA(T21N)-RagC
Plasmid#99644PurposeBacterial expression of RagA(T21N)-RagCDepositorAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pN3FS-CYC1
Plasmid#85783PurposeN-ICE plasmid pH3FS-CYC1DepositorInsertloxP-NatMX-loxP-CYC1p-3FLAG
UseCre/LoxExpressionYeastMutation*(see below)Available sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo
Plasmid#74919PurposeMammalian expression of mKlf2 and mNanog-mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsT2A and mCherryExpressionMammalianPromoterCAGAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
MMRN1-bio-His
Plasmid#53409PurposeExpresses full-length Multimerin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertMMRN1 (MMRN1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA
Plasmid#47446PurposeAAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5HD_C63)-VP64
UseAAV; TaleTags2A_GFPExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Smad1 EVE GM
Plasmid#27997DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman Smad1 EVE Gsk3 mutant (SMAD1 Human)
TagsFlagExpressionMammalianMutationS463E S465E S210A T202A S191A S198AAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor
Plasmid#202689PurposeCLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCopGFP
mCherry
Available sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
cr3Ec
Plasmid#184860Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination systemDepositorInsertCas9, gam, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z18-PDGFR_EF1a_eGFP
Plasmid#186304PurposePlasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ18-PDGFR
eGFP
TagsHA, mycExpressionMammalianAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gene Trap - 24XMS2 Lentiviral Vector
Plasmid#174198PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviralTags24X MS2 hairpins, Blasticidin, and T2AAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dzCas9-NG-Act3.0
Plasmid#158415PurposeCRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRExpressionPlantAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-CAG-hCAS9-neo
Plasmid#166026PurposeAAVS1 targeting vector carrying CAG-SpCas9-neoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAAVS1-CAGCas9IRESneo
ExpressionMammalianAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
INS-2A-luciferase-2A-tdT
Plasmid#159348PurposeHuman INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase-2A-tdTomato
UseIns locus knockin donor vectorAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC059-HisMS2_PLP_NucPhos_pac
Plasmid#155039PurposeGeneration of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein geneDepositorInsertsMaturation Protein
Coat Protein Dimer
Nucleocapsid Phosphoprotein
ExpressionBacterialMutationRemove TypeIIs Restriction SitesPromoterT7Available sinceSept. 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDGE668
Plasmid#153233PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE666
Plasmid#153231PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA
Plasmid#158987PurposeVector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neuronsDepositorInsertdSaCas9-VP160
UseAAV and CRISPRExpressionMammalianPromoterpMecp2Available sinceAug. 29, 2020AvailabilityAcademic Institutions and Nonprofits only