4,183 results

1. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

   Plasmid

   #20943

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available Since

   July 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. RCIscript-GoldyTALEN

   Plasmid

   #38142

   Purpose

   destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

   Depositor

   Daniel Carlson, Stephen Ekker

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

   Insert

   GoldyTALEN
   

   Use

   Mrna transcription vector

   Tags

   AcV5 and FokI homodimer

   Mutation

   Truncate at N and C terminous

   Promoter

   T3

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPGKFLPobpA

   Plasmid

   #13793

   Purpose

   Expression of mouse codon-optimized FLP recombinase with bovine growth hormone polyadenylation (bpA) sequence.

   Depositor

   Philippe Soriano

   Article

   Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

   Insert

   FLPo
   

   Expression

   Mammalian

   Available Since

   Jan. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. MG15-luc (mut p53 binding sites)

   Plasmid

   #16443

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

   Insert

   p53-binding site (mutant) (TP53 )
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET-RCaMP1h

   Plasmid

   #42874

   Purpose

   a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

   Depositor

   Loren Looger

   Article

   Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

   Insert

   Red Fluorescent Calcium binding Protein
   

   Tags

   EK (Enterokinase) Recognition Site, His Tag (6x),…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pyronic/pcDNA3.1(-)

   Plasmid

   #51308

   Purpose

   Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

   Depositor

   Luis Felipe Barros

   Article

   San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

   Insert

   Pyronic
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4-Beclin1 (FL)

   Plasmid

   #24388

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTDH3-dCas9-Mxi1

   Plasmid

   #46921

   Purpose

   Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-Mxi1
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TDH3

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. 12XCSL-DsRedExpressDL

   Plasmid

   #47683

   Purpose

   Notch reporter

   Depositor

   Urban Lendahl

   Article

   Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

   Insert

   TP-1 promoter
   

   Use

   Notch reporter construct

   Tags

   dsRedExpressDR

   Mutation

   hexamerized 50-bp EBNA2 response element of the T…

   Promoter

   NA

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. ankG-mCherry

    Plasmid

    #42566

    Depositor

    Benedicte Dargent

    Article

    Leterrier et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    ankyrin G (Ank3 Rat)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge