We narrowed to 7,755 results for: RAP

Showing: 6881 - 6900 of 7755 results

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IL2-AHDC2-Cherry in pmCherryN1

Plasmid

#187291

Purpose

Express pmCherry-IL2-AH-deltaC2

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Scraps interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2

Promoter

CMV

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

Plasmid

#227800

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA 2 (Vegfa Synthetic)
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3

Plasmid

#227801

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA 3 (Vegfa Synthetic)
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRev-erba-hSyn-mCherry-KASH

Plasmid

#223226

Purpose

guideRNA targeting the mouse Rev-erb alpha (Nr1d1)

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Nr1d1 (Nr1d1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1876-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

Plasmid

#223162

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-LA

Plasmid

#206197

Purpose

Expresses GFP-tagged human Lamin-A protein by the constitutive CMV promoter in mice

Depositor

Yuxuan Guo

Article

Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

Insert

Lmna (LMNA )

Use

AAV

Tags

GFP

Promoter

CMV

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1861-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

Plasmid

#223161

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

Plasmid

#223166

Purpose

2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346

Plasmid

#217012

Purpose

Expression of CDK7 in insect cells

Depositor

Manjeet Mukherjee

Article

Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

Insert

CDK7 (S164D, T170E)_del327-346 (CDK7 Human)

Tags

TEV cleavable his tag

Expression

Insect

Mutation

(S164D, T170E)_del327-346

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac dual_Human full length CDK7(W132R, S164D, T170E)

Plasmid

#217013

Purpose

Expression of CDK7 in insect cells

Depositor

Manjeet Mukherjee

Article

Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

Insert

CDK7(W132R, S164D, T170E) (CDK7 Human)

Tags

TEV cleavable his tag

Expression

Insect

Mutation

CDK7(W132R, S164D, T170E)

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]

Plasmid

#197599

Purpose

Pan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactive

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

Synaptogyrin-CRY2 (D387A+E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)

Expression

Worm

Mutation

D387A, E490G

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-748_HA-GD2-28z_CAR_BATF

Plasmid

#207488

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-805_HA-GD2-28z_CAR_BATF-RFP

Plasmid

#207492

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF-RFP, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-755_HA-GD2-28z_CAR_RFP-JUN

Plasmid

#207494

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-JUN, HA-GD2-28z_CAR (JUN Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-837_NY-ESO-1_TCR_tFAS

Plasmid

#207500

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tFAS, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V1-rCD20-BSD

Plasmid

#209752

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-rCD20-BSD

Plasmid

#209753

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-rCD20-BSD

Plasmid

#209754

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_MED13L_COL23A1

Plasmid

#205849

Purpose

Express mEGFP-tagged fusion protein, MED13L_COL23A1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

MED13L_COL23A1 (MED13L, COL23A1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pExp-His-zBasic-RBD-Avi

Plasmid

#195000

Purpose

Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

Depositor

Marko Hyvönen

Article

Fischer et al Nat Biotechnol. 2025 Jun;43(6):960-970.

Insert

RBD (S SARS-CoV-2)

Tags

Avi-tag and His8-Zbasic

Expression

Bacterial

Promoter

T7lac

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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