We narrowed to 9,085 results for: mel

1. shERK1a-mlpx-puro

   Plasmid

   #65228

   Purpose

   encodes a shRNA against ERK1

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   shRNA against ERK2 (MAPK1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1α1.1-Chronos-tdTomato

   Plasmid

   #122099

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   EF1α (1.1 kb short version)

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

   Plasmid

   #75126

   Purpose

   Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   KMT6 (EZH2 Human)
   

   Use

   Retroviral

   Tags

   None

   Mutation

   SET domain replaced with the SET domain of EZH1

   Available Since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCS2plus-mVenus1-155-GGGS-TDP-43

   Plasmid

   #162611

   Purpose

   Allows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   TARDBP (TARDBP Human)
   

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TMEM230-WT-pEGFP(isoform 1)

   Plasmid

   #99561

   Purpose

   mammalian expression of TMEM230-GFP (isoform 1)

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]

   Plasmid

   #108273

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1α promoter (1.1kb short version)

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]

   Plasmid

   #122103

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   EF1a（1.1kb short version)

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

   Plasmid

   #84485

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1α, 1.1 kb long (short version)

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. JNK2 WT O/E (MAPK9)-pcw107-V5

   Plasmid

   #64617

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAPK9 (transcript variant JNK2-a2) (MAPK9 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   none

   Promoter

   PGK

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

10. FLAG-MKK6 (S207E,T211E)-pcw107

    Plasmid

    #64625

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K6 (MAP2K6 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    S207E,T211E

    Promoter

    PGK

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. myr-FLAG-MEK5-pcw107

    Plasmid

    #64620

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K5 (MAP2K5 Human)
    

    Use

    Lentiviral

    Tags

    Flag and myristoylated

    Promoter

    PGK

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pBRPy CAGGS-Mbd3-IRES-PURO

    Plasmid

    #52376

    Purpose

    Mammalian expression vector for constitutive expression of Mbd3

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Tags

    2xFlag (DYKDDK)

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. NES-EGFP-PH-BTKx1

    Plasmid

    #183651

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Frog, Human)
    

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 2-170

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TMEM230-WT-IRES(isoform 2)

    Plasmid

    #99564

    Purpose

    mammalian expression of TMEM230 (isoform 2) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-CAG-FLPX-rc [ChrimsonR-GFP]

    Plasmid

    #118295

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CAG

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. shERK2b-mlpx-neo

    Plasmid

    #65231

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]

    Plasmid

    #128588

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1α (1.1 kb short version)

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEAHISMRSA0482

    Plasmid

    #96968

    Purpose

    expression of methicillin-resistant Staphylococcus aureus orf 0482

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    MRSA ORF0482
    

    Tags

    N-ter TEV protease cleavable 6His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-YAP2 (8SA)-pcw107

    Plasmid

    #64637

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    YAP2 (transcript variant 3) (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    8SA*

    Promoter

    PGK

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. mRFP-FKBP-INPP5E(D556A)

    Plasmid

    #183678

    Purpose

    Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    INPP5E (INPP5E Human)
    

    Tags

    mRFP-FKBP12(1-108)

    Expression

    Mammalian

    Mutation

    C641A, D556A

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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