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pVL45 - pPXY>>

Plasmid

#116011

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pPXY:GR-LhG4:tPXY:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Use

Tags

HDEL and SP(ER)

Expression

Plant

Mutation

Promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_CD2AP-1/3

Plasmid

#91398

Purpose

Protein expression and purification of human SH3 domain construct CD2AP-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CD2AP-1/3 (CD2AP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_EPS8-1/1

Plasmid

#91254

Purpose

Protein expression and purification of human SH3 domain construct EPS8-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8-1/1 (EPS8 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal W427A

Plasmid

#47349

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Ha

Expression

Mammalian

Mutation

W427A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pHH0103_BAIAP2L2-1/1

Plasmid

#91393

Purpose

Protein expression and purification of human SH3 domain construct BAIAP2L2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BAIAP2L2-1/1 (BAIAP2L2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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HLH Clock Pas 1-401 VenN

Plasmid

#47353

Purpose

Clock fragment cloned and tagged with VenN

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pFLAG-WTS-1

Plasmid

#66856

Purpose

Express FLAG-tagged C.elegans WTS-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

WTS-1 (wts-1 Nematode)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E153G and D871G mutations in WTS1 compared to Gen…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pIDS-RESTmut full-length

Plasmid

#109162

Purpose

Encodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length REST/NRSF (REST Human)

Use

Tags

Expression

Insect

Mutation

Cys402Ser

Promoter

p10

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMextli

Plasmid

#79262

Purpose

Expresses EGFP-tagged DmMextli in S2 cells

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli (mxt NM_164598, Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase delta 55/S1126A

Plasmid

#59825

Purpose

Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

delta 55/S1126A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal V435R

Plasmid

#47350

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Ha

Expression

Mammalian

Mutation

V435R

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pBABE-EGFP-THAP11-rescue-3xFLAG

Plasmid

#37000

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 (THAP11 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Mutations were generated at codons 273-275 conver…

Promoter

Available since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN L74E

Plasmid

#47355

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L74E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_LCK-1/1

Plasmid

#91332

Purpose

Protein expression and purification of human SH3 domain construct LCK-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LCK-1/1 (LCK Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST attB Orco (Y478A)

Plasmid

#73928

Purpose

UAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid change

Depositor

Leslie Vosshall

Article

Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

Insert

Orco (Y478A) (Orco Fly)

Use

Unspecified

Tags

Expression

Mutation

changed 478 Tyrosine for Alanine

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal L150E

Plasmid

#47346

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Ha

Expression

Mammalian

Mutation

L150E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pFastBac-hDcr-K70A.

Plasmid

#89145

Purpose

Expression of helicase mutant of human Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

hDicer (DICER1 Human)

Use

Tags

His6-tev

Expression

Insect

Mutation

K70A

Promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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HLH Bmal Pas 1-465-VenC

Plasmid

#47364

Purpose

Bmal fragment cloned with Venus tag and used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 ATG m7

Plasmid

#52035

Purpose

encodes mutant HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

all seven let-7 sites in the 3′ UTR mutated (‘m7’)

Promoter

CMV

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pGS-BacA-21222-SIN3A-3xFLAG

Plasmid

#109213

Purpose

Encodes human full-length SIN3A with a C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length SIN3A (SIN3A Human)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-HDAC1

Plasmid

#109209

Purpose

Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human, Synthetic)

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-(ΔTP)DM3(Col-0)

Plasmid

#229729

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Promoter

Available since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB515_35S-HA-(ΔTP)DM3(Col-0) D332R

Plasmid

#229719

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-155 were deleted

Promoter

Available since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB515_35S-HA-(ΔTP)DM3(Col-0) ΔC

Plasmid

#229717

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Promoter

Available since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB515_35S-HA-(ΔTP)DM3(Col-0) Q345R

Plasmid

#229720

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

amino acid 1-52 were deleted, Q345R

Promoter

Available since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB515_35S-HA-(ΔTP)DM3(Col-0) D332R Q345R

Plasmid

#229721

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

amino acid 1-52 were deleted, D332R Q345R

Promoter

Available since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB515_35S-HA-(ΔTP)DM3(Hh-0) S214A

Plasmid

#229722

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

amino acid 1-52 were deleted, S214A

Promoter

Available since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB515_35S-HA-(ΔTP)DM3(Col-0) S214A

Plasmid

#229723

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

amino acid 1-52 were deleted, S214A

Promoter

Available since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB515_35S-HA-(ΔTP)DM3(Col-0) T359A

Plasmid

#229727

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

amino acid 1-52 were deleted, T359A

Promoter

Available since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB512_35S-FLAG-(ΔTP)DM3(Hh-0)

Plasmid

#229728

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Promoter

Available since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge