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pcDNA3.0-HA-UbcH13

Plasmid

#12461

Depositor

Dong-Er Zhang

Article

Zou et al Biochem Biophys Res Commun. 2005 Oct 14.

Insert

Ubc13 (UBE2N Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 WT in pcDNA3.1

Plasmid

#118213

Purpose

Expresses HA-UBE2E1 in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Tags

HA

Expression

Mammalian

Mutation

none

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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negMaLionB

Plasmid

#113911

Purpose

negative control for Blue ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionB

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pIBA2-Int-SpyTag

Plasmid

#198038

Purpose

Expression plasmid for producing SpyTagged stalled secretion variant of intimin

Depositor

Jack Leo

Article

Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

Insert

eaeA

Tags

SpyTag

Expression

Bacterial

Mutation

lacks native signal peptide (amino acids 1-39)

Promoter

tet

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCMU-TPr

Plasmid

#61189

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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p201G Cas9

Plasmid

#59178

Purpose

Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
sGFP

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pCN34e-kimA-yfp

Plasmid

#227638

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-HIS-Tol2

Plasmid

#112486

Purpose

functional recombinant Tol2 protein from E.coli

Depositor

Stephen Ekker

Article

Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

Insert

Tol2 Transposase (tpase Synthetic)

Tags

HIS/6x

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Keratin 18 (pcDNA3)

Plasmid

#18064

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pRK792

Plasmid

#8830

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219P mutant

Tags

His, MBP (cleaved), and polyarginine

Expression

Bacterial

Mutation

S219P mutation

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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NeonGreen-C1-ORP2

Plasmid

#220069

Purpose

overexpresses ORP2 with a neonGreen fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL2 (OSBPL2 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsi (Cys)

Plasmid

#109373

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ14101 aeBlue

Plasmid

#239453

Purpose

express aeBlue chromoprotein

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

aeBlue

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGEP179_pX330K

Plasmid

#137882

Purpose

Entry vector for multi-gRNA cloning

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-T7-OmpA-MNaseA-6xHis

Plasmid

#214808

Purpose

Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

Depositor

Peter Sarin

Article

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease A

Tags

6xhis

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pACS129

Plasmid

#204594

Purpose

Histone H3.1-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.1 (H3C3 Human)

Use

Lentiviral

Tags

human histone H3.1-V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM705 Gst (Cys)

Plasmid

#109376

Purpose

GsX chimera with Gαt C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gst chimera

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NeonGreen-C1-ORP6

Plasmid

#220072

Purpose

overexpresses ORP6 with a neonGreen fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL6 (OSBPL6 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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Lyn11-targeted FRB (LDR)

Plasmid

#20147

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

Lyn-linker-FRB (LDR) (MTOR Human)

Expression

Mammalian

Available Since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

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pCYP64

Plasmid

#212897

Purpose

Expresses the Priestia megaterium LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Mutation

P450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…

Promoter

PcymRC

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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