We narrowed to 7,782 results for: CCH

Showing: 6941 - 6960 of 7782 results

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peGFP-HDAC1 (pc2447)

Plasmid

#240148

Purpose

Expression vector containing a fusion protein of eGFP and human HDAC1.

Depositor

Cristina Cardoso

Article

Rausch et al Nucleic Acids Res. 2020 Dec 16;48(22):12

Insert

HDAC1 (HDAC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEasyG2_hph

Plasmid

#184915

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37deltaSEP

Plasmid

#113501

Purpose

Bacterial expression of GST-tagged p37 lacking the SEP domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 140-206

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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mcherry p62 delta UBA silent

Plasmid

#187984

Purpose

mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Lacking UBA domain (aa389-434) but it has the sil…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pUDC175

Plasmid

#103019

Purpose

S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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mcherry-p62 delta PB1 silent

Plasmid

#187985

Purpose

human p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Lacking PB1 domain (aa1-102) with a silent mutati…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU-D261A

Plasmid

#31729

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

Changed D261 to Alanine

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU-E264A

Plasmid

#31730

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

Changed E264 to Alanine

Promoter

None

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pAL005

Plasmid

#233865

Purpose

MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-mCherry-FLARE-AKAR

Plasmid

#123333

Purpose

Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-AKAR

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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ss-SNAP-TMD-NS3-Gal4

Plasmid

#112279

Purpose

Encodes the membrane-targeted TMD-NS3-Gal4, which exhibits drug-inducible transcription "turn-off" activity. SNAP-tag is fused to the extracellular region of the encoded protein construct.

Depositor

John Ngo

Article

Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

Insert

SNAP-TMD-NS3-Gal4

Tags

AU1 (N term on NS3) and HA (C term on NS3)

Expression

Mammalian

Mutation

NS3 mutant T54A

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-acta1:QFGal4-SV40pA

Plasmid

#155121

Purpose

Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

acta1:QFGal4

Use

Zebrafish expression

Promoter

acta1

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU-E257A

Plasmid

#31727

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

Changed E257 to Alanine

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCAGGS-4580NES

Plasmid

#138376

Purpose

Booster-JNK, a Red-Shifted Genetically Encoded FRET Biosensor for JNK activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-JNK

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR

Plasmid

#61624

Purpose

FRET-based biosensor for monitoring Akt activity

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR (AKT1 Human, Budding Yeast, Synthetic)

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pPBC-LG3-tdT

Plasmid

#62810

Purpose

pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.

Depositor

Karen Wilcox

Article

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-GAL-Kan

Plasmid

#232092

Purpose

Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

SpCas9 and Ec86 reverse transcriptase

Use

CRISPR

Expression

Yeast

Mutation

Ec86 reverse transcriptase is codon optimized for…

Promoter

GAL1-GAL10

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pFUPI hT5-S322-Cyp

Plasmid

#80282

Purpose

Expresses human TRIM5 CypA fusion

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

TRIM5 CypA fusion (TRIM5, PPIA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pST1374-GCN4-dCas9

Plasmid

#113023

Purpose

Expresses GCN4-dCas9 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

GCN4-dCas9 (GCN4 Budding Yeast, S. pyogenes)

Expression

Mammalian

Mutation

D10A, D839A, H840A, N863A

Promoter

CMV

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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