We narrowed to 7,672 results for: CCH

1. pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE

   Plasmid

   #120393

   Purpose

   pAAV plasmid expressing Diras2 shRNA1 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

   Insert

   Diras2 shRNA (Diras2 Mouse)
   

   Use

   AAV and RNAi

   Tags

   mCherry.3XFLAG

   Promoter

   U6

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEasyG2_zeo

   Plasmid

   #184913

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEasyG2_nat

   Plasmid

   #184914

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEasyG3_zeo

   Plasmid

   #184917

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pST1374-GCN4-H840A

   Plasmid

   #113024

   Purpose

   Expresses GCN4-H840A in mammalian cells

   Depositor

   Xingxu Huang

   Article

   Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

   Insert

   GCN4-H840A (GCN4 Budding Yeast, S. pyogenes)
   

   Expression

   Mammalian

   Mutation

   D839A, H840A, N863A

   Promoter

   CMV

   Available Since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Venus-Venus-FLARE-AKAR

   Plasmid

   #123331

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-Venus-FLARE-AKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

   Plasmid

   #123342

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-EKAR-EV
   

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

   Plasmid

   #184740

   Purpose

   encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109

   Depositor

   Randall Platt

   Article

   Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

   Insert

   FsRT-Cas1(opt)-Cas2(opt)
   

   Expression

   Bacterial

   Mutation

   sequence codon optimized for expression in E. coli

   Promoter

   pTetA

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST1374-H840A-GCN4

   Plasmid

   #113027

   Purpose

   Expresses H840A-GCN4 in mammalian cells

   Depositor

   Xingxu Huang

   Article

   Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

   Insert

   H840A-GCN4 (GCN4 Budding Yeast, S. pyogenes)
   

   Expression

   Mammalian

   Mutation

   D839A, H840A, N863A

   Promoter

   CMV

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5FRT/TO PP1gamma-2*Strep

    Plasmid

    #113506

    Purpose

    Expresses dual Strep-tagged PP1gamma in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    PP1gamma (PPP1CC Human)
    

    Tags

    Strep-Strep

    Expression

    Mammalian

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mCherry-mCherry-FLARE-EKAR-EV

    Plasmid

    #123341

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-EKAR-EV
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMGF157

    Plasmid

    #96972

    Purpose

    ish1-mCherry insertion cassette with Hygro resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + hygro…

    Promoter

    N/A

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET151-Vps4(101-437)-Hcp1 K321A/R325A

    Plasmid

    #89287

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 and Ar…

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. myc-Med4 pBacPAK8

    Plasmid

    #15364

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    Med4 (MED4 Human, N/A)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

15. HOXB9 (murine) HIS-tag pET

    Plasmid

    #8541

    Depositor

    Corey Largman

    Article

    Chang et al Mol Cell Biol 1996 Apr;16(4):1734-45.

    Insert

    HOXB9 (Hoxb9 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Missing the first 13 amino acids of HOXB9; makes …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pAG415-GPDp-EGFP-rap1-K651R

    Plasmid

    #84536

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K651R

    Promoter

    GPD

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y

    Plasmid

    #60393

    Purpose

    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast, Synthetic)
    

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4)

    Promoter

    GAL

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pMGF170

    Plasmid

    #96997

    Purpose

    ish1-mCherry insertion cassette with NAT resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + NAT r…

    Promoter

    N/A

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-MED9

    Plasmid

    #15408

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    MED9 (Med9 Mouse)
    

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

20. FLAG-Med9 pBacPAK8

    Plasmid

    #15363

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    Med9 (Med9 Mouse)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only