We narrowed to 7,206 results for: RAP

1. FRB-mCherry-EEA1

   Plasmid

   #222419

   Purpose

   To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Tags

   FRB-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-X1-Neo-GFP-ATL1

   Plasmid

   #109018

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL1 (ATL1 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EIF-1a promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGAN201

   Plasmid

   #129204

   Purpose

   yTRAP sensor of [RNQ+], detects aggregation of Rnq1

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   RNQ1 (RNQ1 Synthetic, Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SUP35

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB80-FKBP-mCherry-RAB11

   Plasmid

   #174622

   Purpose

   Rapalog-inducible coupling to recycling endosomes (RAB11) via FKBP

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   FKBP-mCherry-RAB11 (RAB11A Synthetic, Human)
   

   Use

   Tags

   FKBP-mCherry

   Expression

   Mammalian

   Mutation

   mCherry: Met1Del; RAB11: Met1Del

   Promoter

   Chicken beta-actin

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT25_GalL_BE3

   Plasmid

   #143736

   Purpose

   Expresses BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAN200

   Plasmid

   #129203

   Purpose

   yTRAP sensor of [PSI+], detects aggregation of Sup35

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   Sup35NM (SUP35 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   N terminal and middle domains

   Promoter

   SUP35

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 SPASTIN , residues 112-196

   Plasmid

   #180627

   Purpose

   Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 112-196

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FKBP12-CB2x2-mTagBFP2-HAx2

   Plasmid

   #207146

   Purpose

   Tandem calcium-buffer with rapalog dimerizer

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   Calbindin-2 (CALB2 Human)
   

   Use

   Tags

   FKBP12, HA, and mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. BACE2_pCSdest

   Plasmid

   #53915

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   BACE2 (BACE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pLenti-X1-Neo-GFP-ATL2-R244Q

    Plasmid

    #109023

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2-R244Q (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Arg244Gln mutation results in dimerization defect

    Promoter

    EIF-1a promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pminiT-Halo-MED1 donor

    Plasmid

    #197868

    Purpose

    Donor plasmid used for building Halo-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Halotag

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQTEV-GTF2F2

    Plasmid

    #31323

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    general transcription factor IIF (GTF2F2 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. FKBP-YPet-RanGAP*

    Plasmid

    #175245

    Purpose

    Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJW1820

    Plasmid

    #154321

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    GFP^SEC (LoxP)^AID*::3xFLAG
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. alpha1Ddelta-G12-FRB

    Plasmid

    #87454

    Purpose

    rapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCs

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    a1D short variants (Cacna1d Rat)
    

    Use

    Tags

    FRB

    Expression

    Mammalian

    Mutation

    delete KYPAKNTTIALQMLERML

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJW1759

    Plasmid

    #154315

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::TEV::AID*::3xFLAG for NT slot
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPTXcGMPRELUC

    Plasmid

    #68503

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    

    Use

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

    Promoter

    OPTX promoter with cGMPRE

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pHH0103_GTF2F1_TFIIF

    Plasmid

    #110037

    Purpose

    Protein expression and purification of GTF2F1_TFIIF

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GTF2F1_TFIIF (GTF2F1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJW1806

    Plasmid

    #154319

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    linker::3xMyc for NT slot
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJW1805

    Plasmid

    #154318

    Purpose

    CT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    linker::3xMyc for CT slot
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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