We narrowed to 7,549 results for: URE
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Plasmid#169589PurposeTier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).DepositorInserttetracycline-controlled SEAP expression
ExpressionMammalianPromoterTetO7-PCMVmin-2Available SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1042
Plasmid#169620PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH267-Tier1-OTetO7-PCMVmin2-SEAP-env140
Plasmid#169590PurposeTier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR env140 ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRenv140-pA).DepositorInserttetracycline-controlled SEAP expression
ExpressionMammalianPromoterTetO7-PCMVmin-2Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2304-Tier1-Tactb-PPGK-NLuc_TB
Plasmid#169549PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertTactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
ExpressionMammalianPromoterPPGKAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR
Plasmid#169499PurposeTier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)DepositorInsertPRPBSA-driven YPet-P2aA-PuroR
ExpressionMammalianPromoterPRBSAAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1047-Tier1-PhCMV-YPet-p2A-PuroR
Plasmid#169498PurposeTier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).DepositorInsertPCMV-driven YPet-P2aA-PuroR
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-6xmut_U
Plasmid#147732PurposeMammalian Expression of HsNot1iso2-6xmutDepositorInsertHsNot1iso2-6xmut (CNOT1 Human)
ExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]
Plasmid#149427PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot2-del458-482-dsRNAres_U
Plasmid#147716PurposeInsect Expression of DmNot2-del458-482-dsRNAresDepositorInsertDmNot2-del458-482-dsRNAres (Rga Fly)
ExpressionInsectAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-2R-2K+3D
Plasmid#178680PurposeBacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues removed, two Lys removed, three Asp added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-2R+2K+3D (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationtwo Arg residues removed, two Lys added, three As…Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1 (BTLA ITIM)-mGFP
Plasmid#180817PurposeExpression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFPDepositorInsertPD-1 (BTLA ITIM) (PDCD1 Human)
UseLentiviralTagsmEGFPMutationreplaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…PromoterSFFVAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_601-660-GB1_AD
Plasmid#148506PurposeBacterial Expression of DmeIF4G_601-660DepositorInsertDmeIF4G_601-660 (eIF4G1 Fly)
ExpressionBacterialAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-4R-2K+5D
Plasmid#178681PurposeBacterial expression of N-terminally 6His tagged A1-LCD with four Arg residues removed, two Lys removed, five Asp added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-4R+2K+5D (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationfour Arg residues removed, two Lys added, five As…Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVD1 tRNA-gRNA E4-En-1 (GB2243)
Plasmid#160565PurposetRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.DepositorInserttRNA-gRNA position E4-En-1 (Multiplexing Edit)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7gRNA-ttn.1_C3
Plasmid#140866PurposesgRNA synthesis vector for ttn.1_C3 (zebrafish titin .1).DepositorInsertzebrafish ttn.1_C3 sgRNA for in vitro transcription (ttn.1 Synthetic, Zebrafish)
UseIn vitro transcription of sgrnasAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A
Plasmid#162639PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cellsDepositorInsertORF68
TagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids K174, R179, and K182 mutated to alani…PromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_3K
Plasmid#131205Purposemammalian expression, tet RDepositorInsertRIPK2 K209/410/538R (RIPK2 Human)
TagsEGFPExpressionMammalianMutationK209/410/538RPromoterpCMVAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(Q38P) SpHIS5 (pNTI664)
Plasmid#115434PurposeExpresses Q38P RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationQ38P; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(H39L) SpHIS5 (pNTI665)
Plasmid#115435PurposeExpresses H39L RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationH39L; MfeI site in intronPromoterashbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_LC_R191K_R201K_R216K_R254K
Plasmid#104467Purposeexpress MBP hnRNPA2 LC with 4 R to K mutationsDepositorAvailable SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only