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We narrowed to 9,474 results for: BLI

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Showing: 6961 - 6980 of 9474 results

  1. pALS3-Ma-sfGFP-TAG150

    Plasmid
    #212121
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212121-plasmid-map-sequence-id-444186

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  2. pBK-Ma pylRS nitroY/haloY-F5

    Plasmid
    #212125
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.
    Depositor
    Ryan Mehl
    Insert
    M. alvus PylRS 3-NY/HaloY F5
    Expression
    Bacterial
    Mutation
    CTG125CTT, N166A V168S, A223C, W239R
    Promoter
    GlnS (constitutive)
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    212125-plasmid-map-sequence-id-443626

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  3. SARS-CoV-2 Spike-d18

    Plasmid
    #164583
    Purpose
    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.
    Depositor
    Jason Sheltzer
    Insert
    SARS-CoV-2 Spike (S SARS-CoV-2)
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1256-1273
    Promoter
    EF1a
    Available Since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164583-plasmid-map-sequence-id-322362

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  4. pB6-Gfap-T2A-tdTomato-FNF-DTA

    Plasmid
    #140271
    Purpose
    B6J Gfap-T2A-tdTomato allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Gfap (Gfap Mouse)
    Use
    Mouse Targeting
    Tags
    C-terminal fusion of T2A-tdTomato
    Expression
    Mammalian
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    140271-plasmid-map-sequence-id-317910

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  5. ACACA

    Plasmid
    #39083
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACACA (ACACA Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. Hs.KRAS4b G13D

    Plasmid
    #83133
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G13D
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83133-plasmid-map-sequence-id-158096

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  7. pJCX3-ACTG2_R257C

    Plasmid
    #228911
    Purpose
    Expresses mutant R257C smooth muscle actin gamma-2 in human cells
    Depositor
    Roberto Dominguez
    Insert
    Actin gamma 2, smooth muscle (ACTG2 Human)
    Tags
    HIS-FLAG-TEV
    Expression
    Bacterial and Mammalian
    Mutation
    R257C
    Promoter
    CMV
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228911-plasmid-map-sequence-id-457665

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  8. pJCX3-ACTG2_WT

    Plasmid
    #228910
    Purpose
    Expresses wildtype smooth muscle actin gamma-2 in human cells
    Depositor
    Roberto Dominguez
    Insert
    Actin gamma 2, smooth muscle (ACTG2 Human)
    Tags
    HIS-FLAG-TEV
    Expression
    Bacterial and Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228910-plasmid-map-sequence-id-457664

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  9. Hs.KRAS4b G12C

    Plasmid
    #83130
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12C mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12C
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83130-plasmid-map-sequence-id-158093

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  10. AICSDP-69: NPM1-mTagRFP-T

    Plasmid
    #124608
    Purpose
    Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1
    Depositor
    Allen Institute for Cell Science
    Insert
    NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    May 13, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    124608-plasmid-map-sequence-id-244304

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  12. Hs.KRAS4a G12D

    Plasmid
    #83168
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12D
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83168-plasmid-map-sequence-id-158131

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  13. Lamin A (G465D)-mRFP

    Plasmid
    #124271
    Purpose
    Lamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (G465D) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (G465D)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124271-plasmid-map-sequence-id-241962

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  16. pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50456
    Purpose
    Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Insert
    rM3D-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50456-plasmid-map-sequence-id-75403

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  19. 4IKP

    Plasmid
    #60080
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CARM1:JMC049-A06:C226836 (CARM1 Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Mutation
    See comments
    Promoter
    polyhedrin
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid
    #66708
    Purpose
    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
    Depositor
    Charles Gersbach
    Insert
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Mutation
    D10A and H840A
    Promoter
    hUbC
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66708-plasmid-map-sequence-id-449286

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Showing: 6961 - 6980 of 9474 results