We narrowed to 9,391 results for: BLI

1. pQTEV-PEPD

   Plasmid

   #31328

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   peptidase D (PEPD Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET3C-ARL16(CO)-His6

   Plasmid

   #216485

   Purpose

   Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.

   Depositor

   Richard Kahn

   Insert

   ADP-ribosylation factor-like 16 (ARL16 Human)
   

   Tags

   includes GSGSGSHHHHHH (His6) tag at C-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. BASIC_SEVA_66.11

   Plasmid

   #194213

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 26, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Hs.KRAS4a G12C

   Plasmid

   #83167

   Purpose

   Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12C mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12C

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

   Plasmid

   #75295

   Purpose

   An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.

   Depositor

   Brandon Harvey

   Insert

   eGFP-2A-mKv1.2 (Kcna2 Synthetic, Mouse)
   

   Use

   AAV

   Promoter

   human SYN1

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lamin A (R527P)-mRFP

   Plasmid

   #124272

   Purpose

   Lamin A mutant (R527P), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.

   Depositor

   Eric Schirmer

   Insert

   LMNA (R527P) (LMNA Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   LMNA (R527P)

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HSP90B1

   Plasmid

   #39076

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HSP90B1 (HSP90B1 Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pExo1

   Plasmid

   #53357

   Purpose

   Expresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.

   Depositor

   Lingchong You

   Article

   Pai et al Proc Natl Acad Sci U S A. 2012 Nov 27;10

   Inserts

   BlaMs
   hlyAs
   

   Promoter

   p(lac/ara-1)

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-dCas9-DNMT3a-Hygro

   Plasmid

   #104403

   Purpose

   transient expression of dCas9-DNMT3a fusion protein

   Depositor

   Stephan Beck

   Insert

   DNMT3a (DNMT3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lamin A (R482Q)-mRFP

    Plasmid

    #124273

    Purpose

    Lamin A mutant (R482Q), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (R482Q) (LMNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (R482Q)

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. BRAF - A12G in pENTR

    Plasmid

    #216606

    Purpose

    gateway cloning donor vector containing BRAF ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry plasmid

    Mutation

    A12G

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUASp YFP Rac1 DN

    Plasmid

    #52234

    Purpose

    Drosophila expression of YFP-Rac1 dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Rac1 (Rac1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T17N; DN mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid

    #208636

    Purpose

    for E. coli expression of human eIF5B (ENST00000289371)

    Depositor

    Quanfu Ma

    Insert

    human eIF5B (ENST00000289371) (EIF5B Human)
    

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Contain SNP rs1376443, protein sequence not chang…

    Promoter

    T7

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST1K18ms

    Plasmid

    #72646

    Purpose

    Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

    Depositor

    Brian Kvitko

    Article

    Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

    Type

    Empty backbone

    Use

    Sucrose counter-selection allelic exchange vector

    Available Since

    March 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3.1(-) Full length Her2(ERBB2)-Fc

    Plasmid

    #194330

    Purpose

    Mammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion protein

    Depositor

    Alexander McLellan

    Insert

    Her2-Fc (ERBB2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA-GFP

    Plasmid

    #133009

    Purpose

    pFA carrying a sfGFP gene

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    sfGFP
    

    Tags

    6xhis

    Expression

    Bacterial

    Promoter

    tetR

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACP-ADRβ2 Control Plasmid

    Plasmid

    #101128

    Purpose

    Expression of ACP-Tagged β2 Adrenergic Receptor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Beta-2-Adrenergic Receptor (ADRB2 Human)
    

    Tags

    ACP-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

    Plasmid

    #85398

    Purpose

    humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Inserts

    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    

    Use

    AAV

    Tags

    cMyc Tag

    Expression

    Mammalian

    Mutation

    S27A

    Promoter

    human Synapsin1 promotor and ribosomal skip seque…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. HSD17B11

    Plasmid

    #39159

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSD17B11 (HSD17B11 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. Hs.KRAS4a G13D

    Plasmid

    #83170

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G13D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G13D

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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