We narrowed to 11,809 results for: emb
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Plasmid#185685PurposeLow-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERN1 (ERN1 Synthetic, Human)
TagsHaloTagExpressionMammalianMutationDeletion of linker, kinase, and Rnase domainsAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3-K650Q
Plasmid#191756PurposeExpression of N-terminally mEGFP labeled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFGFR3IIIc (FGFR3 Human)
TagsmEGFPExpressionMammalianMutationc.1948A>C (p.K650Q) + silent mutation c.1938C&…Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-hFIP200(1363-1370Mut)-MBP
Plasmid#190038PurposeExpression of FIP200 mutantDepositorInsertFIP200 (RB1CC1 Human)
TagsGST and MBPExpressionMammalianMutation1363 RDKDLIES 1370 to GSSGGSSGPromoterCMVAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A
Plasmid#182047Purposeexpression of Sox2 mutantsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSox2 (Sox2 Mouse)
TagsFLAGExpressionMammalianMutationS248APromoterCAGAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-RHOA_sgRNA
Plasmid#183877PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRHOA sgRNA spacer (RHOA Human)
UseCRISPRExpressionMammalianPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A
Plasmid#182049Purposeexpression of Sox2 mutantsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSox2 (Sox2 Mouse)
TagsFLAGExpressionMammalianMutationS248A/T258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 T258A
Plasmid#182048Purposeexpression of Sox2 mutantsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSox2 (Sox2 Mouse)
TagsFLAGExpressionMammalianMutationT258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-Haunt-EF1a-DsRed
Plasmid#92162PurposeHaunt in trans overexpression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHaunt (Halr1 Mouse)
ExpressionMammalianPromoterCAGAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1ecto&tmd-BTLAicd-mGFP
Plasmid#180794PurposeExpression of human PD-1 extracellular domain and transmembrane domain fused to BTLA intracellular domain followed by mEGFPDepositorAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
TagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP108 Nanog-Venus-2a-mCherry
Plasmid#159743PurposeNanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNanog homeobox (Nanog Mouse)
UseMouse TargetingTagsVenus-2a-mCherryAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/pcDNA3.1
Plasmid#111456PurposeElectrophysiology in HEK cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
ExpressionMammalianMutationKv7.4 (1-645, Δ368-523), C643AAvailable sinceMay 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-GFP
Plasmid#166676PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertsVP2C-GFP
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyExpressionYeastPromoterGAL1 and GAL10Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
px459-RhebL1 sgRNA
Plasmid#133769PurposeExpresses Cas9 and human RhebL1 sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRhebL1 sgRNA
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
phNFL-P440L
Plasmid#132596PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNEFL (NEFL Human)
ExpressionMammalianMutation1319C>T; Pro440LeuPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) C49S and C117S
Plasmid#108913PurposeExpress C49S/C117S double mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7
TagsHalo-TEVExpressionMammalianMutationCysteine 49 to Serine, Cysteine 117 to SerinePromoterSP6Available sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AB (325-557, Δ 368-523)/pET28HMT
Plasmid#111052PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AB (325-557, Δ 368-523)PromoterT7Available sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5-hygro-eGFP-HEC1-8A/S55D
Plasmid#114032PurposeInducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)
ExpressionMammalianMutationHec1 - 8A/S55DAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/181/666A
Plasmid#105310PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKindlin2 (FERMT2 Human)
TagsEGFPExpressionMammalianMutationS159/181/666AAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-zeo: alpha6mGFP
Plasmid#35611Purposemammalian expression of mouse Alpha6 (Chrna6) with internal mEGFP tagDepositorInsertnAChr alpha6mEGFP (Chrna6 Mouse)
TagsmEGFP inserted after A405 with AGA linker on eith…ExpressionMammalianPromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only