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Mito-Lapronic/pGW1

Plasmid

#140754

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Mito-Lapronic

Tags

Mitochondrial Sequence Signal

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGG-TurboID-GFP

Plasmid

#209399

Purpose

Transiently expressing and visualizing the subcellular localization of TurboID in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID

Tags

GFP

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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etnppl-pGEMtEasy

Plasmid

#192263

Purpose

Mouse Etnppl sequence is inserted in pGEM-T Easy vector.

Depositor

Toshihide Yamashita

Article

Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

Insert

ethanolamine phosphate phospholyase (Etnppl Mouse)

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-SunTag

Plasmid

#196920

Purpose

The 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporter

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET19b-rDAD2 cPGFP(G164)

Plasmid

#165994

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rDAD2 cPGFP(G164)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-mBcl11b-MAR4

Plasmid

#178188

Purpose

Lucifease reporter driven by MAR4 regulatory sequences of mouse Bcl11b

Depositor

Katsuhiko Ono

Article

Nomura et al Cell Rep. 2018 Mar 20;22(12):3142-3151.

Insert

Bcl11b MAR4

Use

Luciferase

Expression

Mammalian

Promoter

SV40 promoter

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pmNFH

Plasmid

#85178

Purpose

Mouse neurofilament protein H cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Colakoglu et al J Cell Biol. 2009 Jun 1;185(5):769-77. d

Insert

mouse NFH (Nefh Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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ATM-Halo HRD

Plasmid

#207082

Purpose

Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

None

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pC0.388

Plasmid

#203940

Purpose

Up Flank. Neutral site. Up flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

mrr UP

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.389

Plasmid

#203941

Purpose

Down Flank. Neutral site. Down flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

mrr DOWN

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

Plasmid

#132962

Purpose

Firefly-luciferase single-strand annealing assay cloning backbone

Depositor

Gang Bao

Article

Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

Insert

Firefly Luciferase

Use

CRISPR and Luciferase

Expression

Mammalian

Mutation

Stop sequence between luciferase gene

Promoter

CMV

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Gata3-T2A-H2B-eGFP donor

Plasmid

#113119

Purpose

Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata3 donor region including homology arms and T2A-H2B-eGFP

Use

Synthetic Biology

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-CaNAR2

Plasmid

#64730

Purpose

Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

Lyn-CaNAR2

Tags

Cerulean3, N-terminal targeting sequence from Lyn…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-WNT3A

Plasmid

#108344

Purpose

synchronize trafficking of WNT3A from the ER (RUSH System)

Depositor

David Virshup

Article

Moti et al PLoS One. 2019 Feb 22;14(2):e0212711. do

Insert

WNT3A, Wnt family member 3A (WNT3A Human)

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDonor14-ShoHELIX-KanR (BO4)

Plasmid

#181795

Purpose

pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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YFP-mDia1FH2deltaN

Plasmid

#25419

Depositor

Arthur Alberts

Article

Wallar et al Exp Cell Res. 2007 Feb 1. 313(3):560-71.

Insert

diaph1 (Diaph1 Mouse)

Tags

YFP A206K

Expression

Mammalian

Mutation

mDia1 FH2∆N2 sequence (encodes amino acids 771-11…

Available Since

July 20, 2010

Availability

Academic Institutions and Nonprofits only
Fgfr2-T2A-H2B-Halo donor

Plasmid

#113117

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Fgfr2 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGG-TurboID

Plasmid

#209391

Purpose

Transiently expressing TurboID in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-polg guide

Plasmid

#107598

Purpose

An entry vector with U6a promoter driving polg guide RNA expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

polg guide RNA sequence

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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mito-mGarnet

Plasmid

#104309

Purpose

codes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

mito-mGarnet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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