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Addgene

We narrowed to 866 results for: seph

Showing: 681 - 700 of 866 results
  1. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pGL3 basic Mut E2

    Plasmid
    #48748
    Purpose
    Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)
    Depositor
    Insert
    mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Mutated E-box2 sequence from CACGTT to GCTAGT
    Promoter
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
  3. Per2:iLuc

    Plasmid
    #160293
    Purpose
    target Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.
    Depositor
    Insert
    Per2LUC
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    no promotor
    Available since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pGL3 Pro Mut E2

    Plasmid
    #48750
    Purpose
    Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)
    Depositor
    Insert
    mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Mutated E-box2 sequence from CACGTT to GCTAGT
    Promoter
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
  5. pOPO685

    Plasmid
    #195300
    Purpose
    pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.
    Depositor
    Insert
    McCAST Cascade and native array with PaqCI entry site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    May 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

    Plasmid
    #115137
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS
    Insert
    human codon optimized AcrVA2.1 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 681 - 700 of 866 results