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pCDF-NtXNtT5R1AtR2NtB2
Plasmid#160888PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T5
UseTagsExpressionBacterialMutationPromoterrbcS-T5: T7, chaperones: T11Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT1R1AtR2NtB2
Plasmid#160885PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T1
UseTagsExpressionBacterialMutationPromoterrbcS-T1: T7, chaperones: T8Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS5R1AtR2NtB2
Plasmid#160884PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S5
UseTagsExpressionBacterialMutationPromoterrbcS-S5: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS2R1AtR2NtB2
Plasmid#160883PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S2, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S2: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2+t
Plasmid#48148PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-Y100F
Plasmid#110826PurposeBacterial expression of E.coli DHFR Y100F mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationY100 mutated to F100PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
EC.SurA.pTYB1.a21-281&390-428.L239R
Plasmid#15959DepositorInsertEscherichia coli surA delta PPIase domain 2
UseTagsinteinExpressionBacterialMutationresidues 282-389 deleted; L239R mutationPromoterAvailable sinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLN43 - pBait-1xMS2-7GC[chiP-5']-TtrpA
Plasmid#222404Purposearabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator.DepositorInsert5’UTR of chiP (-98 to +12, relative to AUG) (chiP E. coli)
UseTags1xMS2 hairpin, 7GC clamp, TtrpA terminatorExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYC-yhaM-RFP
Plasmid#207651PurposeOverexpression of native L-cysteine desulfidase and RFP in E. coli MG1655DepositorInsertyhaM
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ7-DadR/DadS-Pdadh-LacZ
Plasmid#191622PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ with transcriptional regulators DadR/DadSDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ9.1-HcdR-Phcdh-LacZ
Plasmid#191629PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ with transcriptional regulator HcdRDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB201
Plasmid#169112PurposeTESEC expression vector for Alr from M. tuberculosisDepositorInsertalr (alr Mycobacterium tuberculosis H37Rv)
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB202
Plasmid#169113PurposeTESEC expression vector for mEGFPDepositorInsertmEGFP
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-xylA
Plasmid#158611PurposeLow phosphate inducible gRNA silencing the xylA promoterDepositorInsertxylA guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1HR1AtR2NtB2
Plasmid#160890PurposeExpression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S1H
UseTags6xHisExpressionBacterialMutationPromoterrbcS-SH1: T7, chaperones: T13Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT4aR1AtR2NtB2
Plasmid#160887PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T4a
UseTagsExpressionBacterialMutationPromoterrbcS-T4a: T7, chaperones: T10Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET15-His-∆NGlpG(Y205F)
Plasmid#155132PurposeExpresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationY205F, insert codes for GlpG residues 87-276PromoterT7Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_M42FDHFR_WTTS
Plasmid#91237Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationM42FPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_M42FG121VDHFR_R166QTS
Plasmid#91238Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationM42FG121 and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_M42FG121VDHFR_WTTS
Plasmid#91239Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationM42FG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_W22HDHFR_R166QTS
Plasmid#91240Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationR166Q and W22HPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_D27NDHFR_R166QTS
Plasmid#91243Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationD27N and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31VDHFR_R166QTS
Plasmid#91245Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31V and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YG121VDHFR_R166QTS
Plasmid#91228Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31YG121 and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YL54IDHFR_R166QTS
Plasmid#91230Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31YL54I and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YL54IDHFR_WTTS
Plasmid#91231Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31YL54IPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_G121VDHFR_R166QTS
Plasmid#91232Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationG121V and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_L54IDHFR_R166QTS
Plasmid#91234Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationL54I and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_L54IDHFR_WTTS
Plasmid#91235Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationL54IPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_M42FDHFR_R166QTS
Plasmid#91236Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationM42F and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m2
Plasmid#84721PurposepVR2 but with a G to U mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationG to U mutation at position 16 of annotated "…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m3
Plasmid#84722PurposepVR2 but with a C to G mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationC to G mutation at position 22 of annotated "…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m1
Plasmid#84720PurposepVR2 but with a C to U mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationC to U mutation at position 13 of annotated "…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys
Plasmid#110541PurposeBacterial protein of E.coli DHFR C85A/C152S mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationC85 mutated to A85, C152 mutated to S152PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCML 313
Plasmid#98586PurposepNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).DepositorInsertcsrB
UseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala13
Plasmid#87174PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proDAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala11
Plasmid#87172PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proAAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala12
Plasmid#87173PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proCAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHL 1410
Plasmid#53011PurposepLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
ompC
rpoS
UseSynthetic BiologyTagsT7noRBS (900bp) and gfpExpressionBacterialMutationcontains −150 to +30 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 762
Plasmid#52990PurposepLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfpDepositorInsertsMicC sRNA
sodB
ompC
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutation−56 to +141 bp and −81 to +36 bpPromoterpLlacO-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1158
Plasmid#53007PurposepLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA
ompC
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains -56 to +141 relative to start codon and …PromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1529
Plasmid#53027PurposePLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st2), RBS(st3), and glgC leader contains -61 …ExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2
Plasmid#48146PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1
Plasmid#48145PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
EC.SurA.pTYB1.a21-281&390-428.M231R
Plasmid#15958DepositorInsertEscherichia coli surA delta PPIase domain 2
UseTagsinteinExpressionBacterialMutationresidues 282-389 deleted; M231R mutationPromoterAvailable sinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCT02
Plasmid#212152PurposePlasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.DepositorInsertFAST-PETase
UseTags6x HisExpressionBacterialMutationS121E, D186H, R224Q, N233K, R280APromoterPT7Available sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZE21-GFPaav
Plasmid#26643DepositorInsertpZE21-GFPaav
UseTagsGFPaavExpressionBacterialMutationnaPromoterAvailable sinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only
