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pCLXSN(GFP)-HA-Tbkbp1

Plasmid

#125177

Purpose

Retroviral expression of mouse Tbkbp1

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHyg-AID(1-114)

Plasmid

#99513

Purpose

Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(1-114) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

Expression

Mutation

internal inversion of nucleotides 265-312 of the …

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Tip alpha K102A/K104A (21-192)

Plasmid

#23159

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Use

Tags

His

Expression

Bacterial

Mutation

changed lysine 102 and lysine 104 to alanine, and…

Promoter

Available since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
LT3GEPIR-WT1shRNA

Plasmid

#220560

Purpose

To inducibly knockdown WT1 or EWSR1::WT1 expression

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

WT1 shRNA (WT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G (TetOP) promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

Plasmid

#205603

Purpose

Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

γ-tubulinWT-RNAi-resistant-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

G2228A, C2231T, C2237T, G2240A

Promoter

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

Plasmid

#205604

Purpose

Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

γ-tubulinΔGTP-RNAi-resistant-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

G2228A, C2231T, C2237T, G2240A, N229A

Promoter

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pFlare9A-CD45i4e5i5mutCBS

Plasmid

#131835

Purpose

A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

PTPRC (PTPRC Human)

Use

Tags

Expression

Mammalian

Mutation

partial intron 4 full exon 5 and partial intron 5…

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(WT)

Plasmid

#112720

Purpose

Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(WT)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHyg-AID(31-114)

Plasmid

#99514

Purpose

Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(31-114) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

Expression

Mutation

internal inversion of nucleotides 265-312 of the …

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM10-HA

Plasmid

#65107

Purpose

Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM10 (ADAM10 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 19-455

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 3xFlag IFIT2

Plasmid

#53555

Purpose

mammalian expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Plasmid

#180474

Purpose

Inducible gene expression vector pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

QFDBD-2xQFAD*-VP16*-EcR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3-MAP4-MTBD

Plasmid

#171498

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

So et al Science. 2019 Jun 28;364(6447). pii: 364

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

mClover3

Expression

Mutation

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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PH-Btk(R28C)-GFP

Plasmid

#51464

Purpose

Non-PIP3 binding control for PH-Btk-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

BTK (aa 1–177) (BTK Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R28C

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pET28a(+) 6xHis-TEV-IFIT2

Plasmid

#53558

Purpose

bacterial expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Use

Tags

6xHIS and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pCK314

Plasmid

#110545

Purpose

Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

Inserts

Modified PrhaBAD (CRP-binding sites removed)
yfp
rhaS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pT5T_hp53_ST

Plasmid

#34922

Depositor

Thanos Halazonetis

Article

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 1-393) (TP53 Human)

Use

Tags

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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p260 pCMV-CRE-M-AR(wt)

Plasmid

#12494

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

CRE-M AR (AR Human)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

CRE-M and AR ligand binding domain

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pCLXSN(GFP)-HA-Tbkbp1 1-280

Plasmid

#125178

Purpose

Retroviral expression of mouse Tbkbp1 (1-280)

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Contains amino acids 1-280

Promoter

CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLXSN(GFP)-HA-Tbkbp1 1-330

Plasmid

#125179

Purpose

Retroviral expression of mouse Tbkbp1 (1-330)

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Contains amino acids 1-330

Promoter

CMV

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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