We narrowed to 2,461 results for: T2A

1. pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

   Plasmid

   #199585

   Purpose

   Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
   AkaLuc
   Blasticidin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-T2A-LexGAD-3xP3-RFP

   Plasmid

   #127558

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   LexGAD
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-T2A-Gal4DBD-3xP3-RFP

   Plasmid

   #127564

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal4DBD
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   July 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV.U6.gDNMT1.Cas9-T2A-GFP

   Plasmid

   #170362

   Purpose

   Plasmid used for Crispr/cas9 based disruption of human DNMT1.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

   Insert

   Cas9-T2A-GFP
   

   Use

   Lentiviral

   Available Since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-UBC-mCD59-T2A-GFP

   Plasmid

   #205451

   Purpose

   lentiviral plasmid for expression of mouse CD59

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mCD59 (Cd59a Mouse)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. P-Ef1a-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170324

   Purpose

   Expresses V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPaint-T2A-p65-3xP3-RFP

   Plasmid

   #127565

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   p65
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. XClone-Cas9-Cronus-T2A-BFP

   Plasmid

   #141209

   Purpose

   Tunable and temporal expression control of Cas9

   Depositor

   Rory Johnson

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-TREX1 g2 Cas9-T2A-mCherry

   Plasmid

   #164251

   Purpose

   Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgTREX1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPaint-T2A-QF2-3xP3-RFP

    Plasmid

    #127559

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    QF2
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-SuperNovaGreen-T2A-dTomato

    Plasmid

    #193798

    Purpose

    Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    SuperNova Green
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-PT2A-GFP-iRFP670-Tdtomato

    Plasmid

    #111813

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    GFP
    iRFP670
    Tdtomato
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1

    Plasmid

    #162098

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to HA-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    HA-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-PT2A-iRFP670-GFP-Tdtomato

    Plasmid

    #111816

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    iRFP670
    GFP
    Tdtomato
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-EF1a-hLMX1A-T2A-copGFP

    Plasmid

    #170444

    Purpose

    Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human LMX1A
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCXLE-dCas9VPH-T2A-GFP-shP53

    Plasmid

    #102895

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPH-T2A-GFP-shP53
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-TREX1 g1 Cas9-T2A-mCherry

    Plasmid

    #164250

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-H2B-sfGFP-T2A-iCre

    Plasmid

    #183351

    Purpose

    Histone H2B-sfGFP and iCre recombinase

    Depositor

    Mario Capecchi

    Insert

    Histone H2B-sfGFP-T2A-iCre
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-EF1a-tetR-T2A-iRFP

    Plasmid

    #176028

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    iRFP
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPaint-T2A-Nluc-3xP3-RFP

    Plasmid

    #127563

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Nluc
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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