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pGGC-LexACasPea

Plasmid

#210539

Purpose

Cloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module C

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

LexA::SpCas9::PEA3AT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS

Plasmid

#191365

Purpose

Overexpression of NLS-dPbaCas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPbaCas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba5Cas13b-EGFP-NLS

Plasmid

#191364

Purpose

Overexpression of NLS-dPba5Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba5Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS

Plasmid

#191363

Purpose

Overexpression of NLS-dPba4Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba4Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS

Plasmid

#191366

Purpose

Overexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dHgm6Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS

Plasmid

#191362

Purpose

Overexpression of NLS-dBba2Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dBba2Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS

Plasmid

#191361

Purpose

Overexpression of NLS-dPba3Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba3Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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DpbdCas12e KRAB

Plasmid

#188505

Purpose

Expresses FLAG tagged DpbdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265H

Plasmid

#149554

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

hAID-ziSpyMacCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ266B

Plasmid

#149555

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

ziSpyMacCas9(D10A)-PmCDA1-UGI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

Plasmid

#133343

Purpose

for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

Plasmid

#133345

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_blaA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-NmCas9-Flag D16A

Plasmid

#109031

Purpose

D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Use

Tags

Flag Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2

Plasmid

#133341

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Availability

Academic Institutions and Nonprofits only

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pX333

Plasmid

#64073

Purpose

Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

3XFLAG-Cas9

Expression

Mammalian

Mutation

Promoter

CBh; U6

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pY010 (pcDNA3.1-hAsCpf1)

Plasmid

#69982

Purpose

Expresses humanized AsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hAsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pY004 (pcDNA3.1-hFnCpf1)

Plasmid

#69976

Purpose

Expresses humanized FnCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hFnCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pME-Cas9-T2A-GFP

Plasmid

#63155

Purpose

Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Insert

Cas9-T2A-GFP

Use

CRISPR; Tol2 middle entry vector for gateway

Tags

Expression

Mutation

Promoter

Available since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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SpRY Cas9

Plasmid

#188491

Purpose

Expresses FLAG tagged SpRY Cas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pY016 (pcDNA3.1-hLbCpf1)

Plasmid

#69988

Purpose

Expresses humanized LbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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