We narrowed to 2,540 results for: organization Addgene

1. pCS2- mRuby-Rab35

   Plasmid

   #194338

   Purpose

   Expresses mRuby Rab35 in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

   Insert

   Rab35 (RAB35 Human)
   

   Tags

   mRuby

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. miniSOG-ER-5

   Plasmid

   #57766

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   ER (CALR Human)
   

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV-DYRK1A-2A-mCherry

   Plasmid

   #118272

   Purpose

   Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
   

   Use

   AAV

   Tags

   2A peptide and mCherry

   Expression

   Mammalian

   Promoter

   CMV with beta global intron

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

   Plasmid

   #140163

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Synthetic, Human)
   

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCOTS-pyl-GFP(35TAG)

   Plasmid

   #92047

   Purpose

   This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

   Depositor

   Lital Alfonta

   Article

   Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

   Inserts

   EGFP
   Pyrrolysyl tRNA(cua)
   Pyrrolysyl tRNA synthetase (methanosarcina mazei)
   

   Use

   Replicative expression plasmid for cyanobacteria …

   Tags

   Histag

   Mutation

   Tyrosine in position 35 of the GFP was mutated f…

   Promoter

   LeuP (native S. elongatus promoter), PpsbII, and …

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5 Myc-tagged PSK

   Plasmid

   #197149

   Purpose

   Expression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

   Insert

   TAOK2 (TAOK2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Wild Type

   Promoter

   SV40

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-DYRK1A(K188R)-2A-mCherry

   Plasmid

   #177166

   Purpose

   Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
   

   Use

   AAV

   Tags

   2A peptide and mCherry

   Expression

   Mammalian

   Mutation

   Changed Lysine 188 to Arginine

   Promoter

   CMV with beta globin intron

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. XIAP (pBS0852)

   Plasmid

   #185282

   Purpose

   For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   XIAP (XIAP Human)
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 2PH-PLCd-APEX2-P2A-mKate2

   Plasmid

   #71284

   Purpose

   Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   2PH-PLCd-APEX2-P2A-mKate2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVC-DNAJB6b

    Plasmid

    #175065

    Purpose

    expresses mVenus and mCherry tagged DNAJB6b in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    DNAJB6b (DNAJB6 Human)
    

    Tags

    V5 and mVenus + mCherry

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HER3-DMVF-181-R

    Plasmid

    #87621

    Purpose

    C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    HER3-DMVF181-C (ERBB3 Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Venus-173-N-ORAI1

    Plasmid

    #87618

    Purpose

    N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus173-N-ORAI1 (ORAI1 Synthetic, Human)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. miniSOG-Golgi-7

    Plasmid

    #57768

    Purpose

    Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Golgi (B4GALT1 Human)
    

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. iMAP-61

    Plasmid

    #187460

    Purpose

    gRNA array of iMAP-61

    Depositor

    Tian Chi

    Article

    Liu et al Cell. 2022 Aug 4;185(16):3008-3024.e16.

    Insert

    sgRNA array
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    modified U6

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HER2-DMVF-181-N

    Plasmid

    #87622

    Purpose

    N-terminal Dronpa fragment fused to the C-terminus of full-length HER2; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    HER2-DMVF181-N (ERBB2 Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pST_DSUP_RAMVA (pBS0353)

    Plasmid

    #185189

    Purpose

    For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DSUP_RAMVA
    

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. miniSOG-LaminB1-10

    Plasmid

    #57771

    Purpose

    Localization: Nuclear Envelope, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    miniSOG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV-DCX(S306D)-2A-mCherry

    Plasmid

    #118292

    Purpose

    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acid

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    Doublecortin (DCX Human)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    Serine 306 changed to Aspartic Acid

    Promoter

    CMV with beta global intron

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-DCX(S306A)-2A-mCherry

    Plasmid

    #118291

    Purpose

    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanine

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    Doublecortin (DCX Human)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    changed Serine 306 to Alanine

    Promoter

    CMV with beta global intron

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS423-mitoT

    Plasmid

    #174525

    Purpose

    Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

    Depositor

    Oleh Khalimonchuk

    Article

    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

    Insert

    Yeast mitochondrial intermembrane tether
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Yeast

    Promoter

    MET25

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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