We narrowed to 9,085 results for: mel

1. pLT3REVIR_MARK3 1122

   Plasmid

   #107232

   Purpose

   shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Insert

   MARK3 shRNA (22 nt) (MARK3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. P3-dCas9-Tet1-GFP-Puro

   Plasmid

   #190728

   Purpose

   Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   3XFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   pCAG

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CD74-ROS1 nonmutant

   Plasmid

   #183813

   Purpose

   Express CD74-ROS1 fusion (C6;R34) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

   Inserts

   ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
   CD74 molecule (CD74 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of CD74 exon 1-6 and ROS1 exon…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CD74-ROS1 G2032R

   Plasmid

   #183814

   Purpose

   Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

   Inserts

   ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
   CD74 molecule (CD74 Human)
   

   Use

   Retroviral

   Tags

   The fusion protein of CD74 exon 1-6 and ROS1 exon…

   Expression

   Mammalian

   Mutation

   G2032R

   Promoter

   CMV

   Available Since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-Flag-SPRTN-E112A

   Plasmid

   #110215

   Purpose

   Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

   Depositor

   Ivana Marinovic-Terzic, Kristijan Ramadan

   Article

   Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

   Insert

   SPRTN (SPRTN Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E112A, P296L (see depositor comments below)

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. delta PE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52380

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Expression

   Mammalian

   Mutation

   deleted -1620 - -1011 upstream from OCT4 ATG

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. delta DE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52381

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Expression

   Mammalian

   Mutation

   deleted -3194 - -1768 upstream from OCT4 ATG

   Promoter

   OCT4

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. mouse deltaPE Oct4-GFP transgenic reporter

   Plasmid

   #52382

   Purpose

   BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP pA
   Oct4 (Pou5f1 Mouse)
   PGK-gb2-Neo
   

   Expression

   Mammalian

   Mutation

   deleted -300 - -1300bp fragment upstream from Oct…

   Promoter

   Oct4 and PGK+gb2

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CD74-ROS1 L2086F

   Plasmid

   #183816

   Purpose

   Express CD74-ROS1 fusion (C6;R34) harboring ROS1 L2086F mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

   Inserts

   ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
   CD74 molecule (CD74 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of CD74 exon 1-6 and ROS1 exon…

   Expression

   Mammalian

   Mutation

   L2086F

   Promoter

   CMV

   Available Since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZL Sox2

    Plasmid

    #26351

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-CD74-ROS1 L2000V

    Plasmid

    #183815

    Purpose

    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 L2000V mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Mutation

    L2000V

    Promoter

    CMV

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE FoxE1

    Plasmid

    #26350

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    FOXE1 (FOXE1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pWZL Sox2 R74P

    Plasmid

    #26355

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    R74P

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pHIV-NAT-FLAG-CIP2A ∆NES (del561-625)

    Plasmid

    #222625

    Purpose

    Lentiviral vector that expresses Flag-tagged CIP2A NES mutant in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of the residues 561-625 (ΔNES)

    Promoter

    EF-1-alpha promoter

    Available Since

    July 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

    Plasmid

    #183078

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    V600E (T>A), S607S (TCC>AGT)

    Promoter

    None for primary insert. PGK for puromycin resist…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Split 'n coexpress CUL3-RBX1

    Plasmid

    #52293

    Purpose

    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Inserts

    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    

    Tags

    GST, IRES, and TEV

    Expression

    Bacterial

    Mutation

    contains aa 1-309, contains aa 310-768, and conta…

    Promoter

    IRES and tac

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pMO14

    Plasmid

    #235736

    Purpose

    Expression of human VPS34 complex II in mammalian cells

    Depositor

    Roger Williams

    Article

    Mercer et al EMBO J. 2021 Jul 15;40(14):e105985. doi:

    Inserts

    UVRAG (UVRAG Human)
    EGFP
    BECN1 (BECN1 Human)
    PIK3C3 (PIK3C3 Human)
    PIK3R4 (PIK3R4 Human)
    

    Tags

    2xStrep-tagII-2xTEV-ZZ

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-EF1a-HIF2a dPA (P405A, P531A)

    Plasmid

    #232954

    Purpose

    Stably express a constitutively stable version of HIF2a (HIF2a dPA)

    Depositor

    William Kaelin

    Article

    Jiang et al Cell. 2025 Feb 25:S0092-8674(25)00156-4.

    Insert

    HIF2a (EPAS1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    P405A, P531A

    Promoter

    EF1a

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

    Plasmid

    #183080

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    -124C>T promoter, Exon 1 C7C (C21>T21) sile…

    Promoter

    None for primary insert (Though partial TERT prom…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

    Plasmid

    #183081

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    -146C>T promoter, Exon 1 C7C (C21>T21) sile…

    Promoter

    None for primary insert (Though partial TERT prom…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge