12,469 results

1. AiP949-Slc17a7-IRES2-FlpO-WPRE-neo

   Plasmid

   #181776

   Purpose

   targeting vector

   Depositor

   Tanya Daigle

   Insert

   Slc17a7 homology arms, FlpO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG1.1-Armc12 delta ARM/FLAG

   Plasmid

   #180496

   Purpose

   Expression vector of ARM domain deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

   Insert

   armadillo repeat containing 12 (Armc12 Mouse)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   ARM domain of Armc12 is deleted

   Promoter

   CAG promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-Armc12/FLAG/BioID2/HA

   Plasmid

   #180498

   Purpose

   Expression vector of BirA-fused mouse Armc12. HA-tagged BirA was linked to the FLAG-tagged Armc12. See Fig.S6 of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

   Insert

   armadillo repeat containing 12 (Armc12 Mouse)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG1.1-Armc12 delta N/FLAG

   Plasmid

   #180495

   Purpose

   Expression vector of N-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

   Insert

   armadillo repeat containing 12 (Armc12 Mouse)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   N-terminus of Armc12 is deleted

   Promoter

   CAG promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG1.1-Armc12/FLAG

   Plasmid

   #180494

   Purpose

   Expression vector of mouse armadillo repeat containing 12 (Armc12) tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

   Insert

   armadillo repeat containing 12 (Armc12 Mouse)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG1.1-Armc12 delta C/FLAG

   Plasmid

   #180497

   Purpose

   Expression vector of C-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

   Insert

   armadillo repeat containing 12 (Armc12 Mouse)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   C-terminus of Armc12 is deleted

   Promoter

   CAG promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AiP937-Gpr139-IRES2-FlpO-WPRE-neo

   Plasmid

   #181768

   Purpose

   targeting vector

   Depositor

   Tanya Daigle

   Insert

   Gpr139 homology arms, FlpO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AiP936-Chrna6-IRES2-FlpO-WPRE-neo

   Plasmid

   #181766

   Purpose

   targeting vector

   Depositor

   Tanya Daigle

   Insert

   Chrna6 homology arms, FlpO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AiP944-Ctxn3-IRES2-FlpO-neo

   Plasmid

   #181770

   Purpose

   targeting vector

   Depositor

   Tanya Daigle

   Insert

   Ctxn3 homology arms, FlpO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AiP940-Sncg-IRES2-FlpO-neo

    Plasmid

    #181773

    Purpose

    targeting vector

    Depositor

    Tanya Daigle

    Insert

    Sncg homology arms, FlpO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT5T_Rev7_1-211_SA112_KVK_YAK

    Plasmid

    #105630

    Purpose

    Expresses mouse monomeric Rev7 protein with mutations abolishing binding to Champ1 and Rev3

    Depositor

    Thanos Halazonetis

    Insert

    Rev7 (Mad2l2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, I41Y, V132K, C133V, A135K,…

    Promoter

    T7

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT5T_Rev7_1-211_SA112_KVK

    Plasmid

    #105634

    Purpose

    Expresses mouse monomeric Rev7 protein

    Depositor

    Thanos Halazonetis

    Insert

    Rev7 (Mad2l2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Promoter

    T7

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid

    #105635

    Purpose

    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid

    Depositor

    Thanos Halazonetis

    Inserts

    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

    Plasmid

    #105637

    Purpose

    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid

    Depositor

    Thanos Halazonetis

    Inserts

    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Promoter

    T7

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT5T_Rev7_1-211_SA112_KVK_Champ1_328-355

    Plasmid

    #105638

    Purpose

    Expresses mouse monomeric Rev7 and fragment of Champ1 protein (amino acids 328-355) from bicistronic plasmid

    Depositor

    Thanos Halazonetis

    Inserts

    Rev7 (Mad2l2 Mouse)
    Champ1 (CHAMP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Promoter

    T7

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmPlum-histone H3

    Plasmid

    #179700

    Purpose

    visualization of nucleus/chromosome

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Mol Biol 2016, 5:2

    Insert

    Histone H3 (Hist1h3h Mouse)
    

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EGFP.T2A.NCLX.3'UTR

    Plasmid

    #181872

    Purpose

    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Inserts

    solute carrier family 8 member B1 (Slc8b1 Mouse)
    EGFP
    3'UTR of solute carrier family 8 member B1
    

    Use

    AAV and Adenoviral

    Tags

    HA, T2A, and myc

    Expression

    Mammalian

    Mutation

    includes a large portion of the Slc8b1 3'UTR…

    Promoter

    synthetic hybrid CAG promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)

    Plasmid

    #170488

    Purpose

    SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

    Insert

    FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV.CBA.YFP.miR-E_shRps6ka2-3

    Plasmid

    #180390

    Purpose

    Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-3 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shRps6ka2-3 (Rps6ka2 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV.CBA.YFP.miR-E_shRps6ka2-2

    Plasmid

    #180389

    Purpose

    Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shRps6ka2-2 (Rps6ka2 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV.CBA.YFP.miR-E_shRps6ka2-1

    Plasmid

    #180388

    Purpose

    Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shRps6ka2-1 (Rps6ka2 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV.CBA.YFP.miR-E_shAtxn1

    Plasmid

    #180387

    Purpose

    Producing AAV that encodes mouse Atxn1 shRNA with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shAtxn1 (Atxn1 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a Cdk2ap1ΔN (MT2B2)

    Plasmid

    #178034

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    First N-Terminal 27aa are deleted

    Promoter

    T7LacI

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLV Pigb-V5

    Plasmid

    #175160

    Purpose

    Lentiviral expression of mouse Pigb-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Pigb (Pigb Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV V5-Chpt1

    Plasmid

    #175155

    Purpose

    Lentiviral expression of V5-tagged mouse Chpt1

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Chpt1 (Chpt1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV Tmx4-V5 C209A

    Plasmid

    #175140

    Purpose

    Lentiviral expression of V5-tagged mouse Tmx4

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmx4 (Tmx4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    C209A

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV Tmx4-V5 C211A

    Plasmid

    #175139

    Purpose

    Lentiviral expression of V5-tagged mouse Tmx4

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmx4 (Tmx4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    C211A

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HisGB1-Fusion + CBP TAZ1(340-439)

    Plasmid

    #173762

    Purpose

    Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid

    #173763

    Purpose

    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid

    #173764

    Purpose

    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge