We narrowed to 9,184 results for: Pol;

1. pDONOR-MARINA

   Plasmid

   #223328

   Purpose

   DONOR vector for genomic integration of MARINA voltage sensitive indicator gene from Platisa et al., 2017 (10.1021/acschemneuro.6b00234).

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Inserts

   MARINA
   KIR2.1 channel Golgi-to-plasma membrane trafficking signal
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mCherry and super-ecliptic pHluorin

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFBOH-MHL.BMI1

   Plasmid

   #224711

   Purpose

   Expresses N-terminal hexahistidine-tagged human BMI1 (PCGF4) in insect cells.

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   BMI1 (BMI1 Human)
   

   Tags

   6x histidine tag

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Unfused click editor construct; mSA-ePhi29 - pCMV-T7-mSA-Phi29 (JO1411)

   Plasmid

   #217807

   Purpose

   Unfused click editor construct expressing mSA-ePhi29(D169A), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   mSA-linker-SV40NLS-ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)

   Plasmid

   #217796

   Purpose

   Variant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Cdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

   Plasmid

   #217797

   Purpose

   Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CILK1-R272Q

   Plasmid

   #214911

   Purpose

   expression of the mutant variant of CILK1

   Depositor

   Pavel Krejčí

   Article

   Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

   Insert

   CILK1 (CILK1 Human)
   

   Tags

   Myc-FLAG

   Expression

   Mammalian

   Mutation

   R272Q substitution

   Promoter

   CMV

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CILK1-E80K

   Plasmid

   #214910

   Purpose

   expression of the mutant variant of CILK1

   Depositor

   Pavel Krejčí

   Article

   Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

   Insert

   CILK1 (CILK1 Human)
   

   Tags

   Myc-FLAG

   Expression

   Mammalian

   Mutation

   E80K substitution

   Promoter

   CMV

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-lox-inv[Talpha1-HA-Dre]-lox-Akna-mOrange2

   Plasmid

   #196893

   Purpose

   Neuron-specific expression of Akna fused to mOrange2. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Akna-mOrange2

   Plasmid

   #196880

   Purpose

   Neuron-specific expression of the centrosomal protein Akna fused to mOrange2

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-128-425-Akap9

    Plasmid

    #196897

    Purpose

    Neuron-specific expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to 2xHA-tags.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    2xHA-Akap9(128-425) (Akap9 Rat)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

    Plasmid

    #196894

    Purpose

    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    N-gTBD-mOrange2 (NEDD1 Human)
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-lox-inv[Talpha1-Dre-PA]-lox-2xHA-CAMSAP3

    Plasmid

    #196895

    Purpose

    Neuron-specific expression of Calmodulin Regulated Spectrin Associated Protein Family Member 3 (CAMSAP3) fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    2xHA-CAMSAP3 (Camsap3 Mouse)
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBactin-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196855

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid

    #196856

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    

    Expression

    Mammalian

    Mutation

    Lacking the centrosome-targeting domain (CTD)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid

    #191099

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-mScarlet-I
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    optimized to human codon usage

    Promoter

    UbC

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ATX3 13Q- I77K Q78K W87K

    Plasmid

    #185908

    Purpose

    Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Human, Synthetic)
    

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Point mutations in Atx3 gene I77K Q78K and W87K

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX2T-TEV CHMP1B 186-199 L188A/L192A

    Plasmid

    #108288

    Purpose

    Expresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed L 188 to A, changed L 192 to A

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX2T-TEV CHMP1B 186-199 L192A/L195A

    Plasmid

    #108290

    Purpose

    Expresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed L 188 to A, changed L 192 to A

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKHH030_sgAMBRA1#2

    Plasmid

    #174147

    Purpose

    Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA (AMBRA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKHH030_sgAMBRA1#1

    Plasmid

    #174146

    Purpose

    Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA (AMBRA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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