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177,602 results

Showing: 7021 - 7040 of 177602 results

  1. pOPINE GFP nanobody:Halo:His6

    Plasmid
    #111090
    Purpose
    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.
    Depositor
    Lennart Wirthmueller
    Publication
    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:
    Insert
    GFP Nanobody
    Tags
    Halo and His6
    Expression
    Bacterial, Insect, and Mamm…
    Promoter
    T7 promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    111090-plasmid-map-sequence-id-264731

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  8. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

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  10. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Publication
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  11. pCMV-DeAct-GS1

    Plasmid
    #89445
    Purpose
    Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-GS1 (GSN Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning cytoplasmic Gsn amino acids 5…
    Promoter
    CMV
    Available since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    89445-plasmid-map-sequence-id-177128

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  13. AICSDP-10:LMNB1-mEGFP

    Plasmid
    #87422
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    6 Articles
    87422-plasmid-map-sequence-id-173949

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  18. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Publication
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1-a and hU6
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    70683-plasmid-map-sequence-id-217910

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Showing: 7021 - 7040 of 177602 results