159,530 results

1. pCAS

   Plasmid

   #60847

   Purpose

   Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

   Depositor

   Jamie Cate

   Article

   Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

   Inserts

   S. pyogenese Cas9
   RNA pol III promoter (tRNA-Tyr)
   hepatitis delta virus ribozyme, genomic
   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS/His8 and TTT 3' extension prior to sgRNA

   Expression

   Bacterial and Yeast

   Mutation

   L4 is UUCG tetraloop and guide targets LYP1 (CATA…

   Promoter

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-ND1 Left -GSVG-UGI

   Plasmid

   #187412

   Purpose

   human mitochondrial DNA ND1 target TALE

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

   Insert

   DddA GSVG variant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hC73S-pEGFP-N1

   Plasmid

   #111536

   Purpose

   Expresses human KCNB1 C73S in mammalian cells for imaging

   Depositor

   Federico Sesti

   Insert

   human C73S-Kv2.1 (KCNB1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C73S

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti6-GFP

   Plasmid

   #35637

   Depositor

   Daniel Haber

   Article

   Singh et al Cell. 2012 Feb 17;148(4):639-50.

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pBIG1a

   Plasmid

   #80611

   Purpose

   pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP Kindlin2

   Plasmid

   #105305

   Purpose

   To study effect of Kindlin 2 on Cell/ECM adhesion

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYX-C-mScarlet-Zeo-TSC22D2

   Plasmid

   #222670

   Purpose

   endogenously tag TSC22D2 with C-terminal mScarlet

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   TSC22D2 (TSC22D2 Human)
   

   Use

   Tags

   mScarlet

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-EGFP

   Plasmid

   #21181

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

   Insert

   enhanced green flourescent protein
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-SEpHluorin

   Plasmid

   #32001

   Depositor

   Sergio Grinstein

   Article

   Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

   Insert

   mCherry
   

   Use

   Tags

   Superecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL

    Plasmid

    #111394

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis, Myc, T7, and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223_PER1_WT

    Plasmid

    #82152

    Purpose

    Gateway Donor vector containing PER1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PER1 (PER1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. IF-GFP-ATRX

    Plasmid

    #45444

    Purpose

    ATRX expression vector

    Depositor

    Michael Dyer

    Article

    Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

    Insert

    ATRX (ATRX Human, Mouse)
    

    Use

    Tags

    GFP and HA

    Expression

    Bacterial and Mammalian

    Mutation

    isoform 2, missing codon E124

    Promoter

    PCMV

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLR5-CAG-NLS-SNAP

    Plasmid

    #174879

    Purpose

    PiggyBac transposon vector for expression of NLS-SNAP

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    NLS-SNAP
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLIB

    Plasmid

    #80610

    Purpose

    pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMD.RVG.CVS24-N2c

    Plasmid

    #19712

    Depositor

    Manfred Schubert

    Article

    Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

    Insert

    Rabies virus CVS24-N2c glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQE30-His-AsnRS

    Plasmid

    #124105

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Asparaginyl-tRNA ligase (asnS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET16b-His-ArgRS

    Plasmid

    #124104

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Argin+I4 ligase (argS Escherichia coli)
    

    Use

    Tags

    10x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPlatTET-gRNA2

    Plasmid

    #82559

    Purpose

    All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMM403_CaMKII-promoter

    Plasmid

    #34926

    Depositor

    Mark Mayford

    Insert

    CaMKII promoter (Camk2a Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge