165,637 results

1. pcDNA3-pmEKAR4

   Plasmid

   #174440

   Purpose

   Plasma membrane-targeted EKAR4.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   pmEKAR4
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-pHluorin_M153R-CD63

   Plasmid

   #172117

   Purpose

   Fluorescent reporter for CD63-positive exosome secretion

   Depositor

   Alissa Weaver

   Article

   Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

   Insert

   CD63 (CD63 Human)
   

   Use

   Lentiviral

   Tags

   pHluorin_M153R in a small extracelluar loop of CD…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-polyQ74-NLS

   Plasmid

   #231892

   Purpose

   Forms nuclear HTT partial exon 1 Q74-NLS aggregates

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q74-NLS (HTT Human)
   

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   CAG repeat expansion

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBWB424

   Plasmid

   #140638

   Purpose

   RSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.

   Depositor

   Keith Tyo

   Article

   Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

   Insert

   Cas9
   

   Expression

   Bacterial

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

   Plasmid

   #225708

   Purpose

   Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

   Depositor

   Michael Lin

   Article

   Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

   Has Service

   AAV9

   Insert

   ASAP5-Kv2.1
   

   Use

   AAV

   Promoter

   EF1α

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-mStayGold-EFS-Puro

   Plasmid

   #227256

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. rAAV9-CAP-in-cis-Lox-AAV

   Plasmid

   #205990

   Purpose

   rAAV9 cap acceptor vector for CREATE libraries

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   rAAV9 cap acceptor vector for CREATE libraries
   

   Use

   AAV

   Mutation

   Deletion between XbaI and AgeI sites

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LRG2.1_Neo

   Plasmid

   #125593

   Purpose

   Lentiviral expression of sgRNA with GFP and neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-HA-mSTING

   Plasmid

   #214157

   Purpose

   Transient expression of STING

   Depositor

   Nan Yan

   Article

   Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

   Insert

   STING (Sting1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFBP_2xNbLYS1::LUZ

    Plasmid

    #189918

    Purpose

    Binary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.

    Depositor

    Adam Steinbrenner

    Article

    Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    Various

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX_305

    Plasmid

    #41390

    Purpose

    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

    Depositor

    David Root

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEMG-GRIS

    Plasmid

    #225134

    Purpose

    For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. THBS1-bio-His

    Plasmid

    #53417

    Purpose

    Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    THBS1 (THBS1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pM1s3AsG

    Plasmid

    #137921

    Purpose

    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    Superfolder GFP
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET_RTX-6xHis

    Plasmid

    #145029

    Purpose

    pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19/PLIN2-GFP

    Plasmid

    #202194

    Purpose

    knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP knock-in

    Promoter

    lac

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-SopB

    Plasmid

    #183657

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1 Full length Importin beta

    Plasmid

    #106941

    Purpose

    Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

    Depositor

    Patrizia Lavia

    Article

    Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

    Insert

    human Importin beta-1 (KPNB1) (KPNB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 - hSRSF6

    Plasmid

    #120419

    Purpose

    overexpression of human SRSF6

    Depositor

    Gillian Bates, Andreas Neueder

    Article

    Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

    Insert

    SRSF6 (SRSF6 Human)
    

    Expression

    Mammalian

    Promoter

    CMV immediate/early

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPR-PE-bacteria

    Plasmid

    #172715

    Purpose

    Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    Cas9n-linker-M-MLV2
    

    Expression

    Bacterial

    Mutation

    H840A of Cas9

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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