We narrowed to 16,357 results for: Jun

1. pCMV-dLight1.3a

   Plasmid

   #111055

   Purpose

   Expresses dLight1.3a in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.3a
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-mCherry-TSC2(AA)-NLS

   Plasmid

   #192443

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

   Plasmid

   #90014

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Ago2
   NBirA*-TNRC6C
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX PLCg1(C)-SH2

   Plasmid

   #46469

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   PLCg1(C)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 656-766

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro

   Plasmid

   #192684

   Purpose

   Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human IL1RN activating gRNAs #1,2,3 (IL1RN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET-pr-mKikGR1 (mKikGR1-V69T)

   Plasmid

   #99227

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mKikGR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   monomeric variant with mutation V69T (Eos notatio…

   Promoter

   T7

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. RNAseq_cal_Tuschl

   Plasmid

   #59426

   Purpose

   Encodes sequence non-cognate to human genome for generation of RNAseq calibrators

   Depositor

   Thomas Tuschl

   Article

   Max et al Proc Natl Acad Sci U S A. 2018 Jun 5;115

   Insert

   RNAseq_cal_Tuschl
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDendra2-Hygro

   Plasmid

   #202407

   Purpose

   Dendra2 expression plasmid

   Depositor

   Morito Kurata

   Article

   Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

   Insert

   Hygromycin
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LSB-hsa-miR-516b-5p

   Plasmid

   #103601

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-516b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-516b-5p target
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG

    Plasmid

    #154777

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    102TAA 150TAG in GFP reporter, hybrid PylT with …

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. YFP-Stim1-ΔCTD

    Plasmid

    #186621

    Purpose

    Encodes Stim1 with C-terminal domain deletion fused to YFP

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Stromal Interaction Molecule 1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal domain 601 to 685

    Promoter

    CMV

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3 Flag MKK7a1 (K76A)

    Plasmid

    #14541

    Depositor

    Roger Davis

    Article

    Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

    Insert

    MKK7a1(K76A) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Lys-76Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

13. mEos3.2trCD4o

    Plasmid

    #203751

    Purpose

    Encodes the transmembrane domain from CD4, including its 2 palmitoylation sites, with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD4o (CD4 Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP

    Plasmid

    #90012

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C
    NBirA*-GFP
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LSB-hsa-miR-30a-3p

    Plasmid

    #103409

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-30a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-30a-3p target (MIR30A Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330_ACTB-i1 sgRNA / hSpCas9

    Plasmid

    #172825

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCXLE-hK + E2-Crimson

    Plasmid

    #74946

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Klf4 (KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pGEX Syk(NC)-SH2

    Plasmid

    #46521

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Syk(NC) (SYK Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 7-263

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEMHE-TNKS-2

    Plasmid

    #154149

    Purpose

    For making TNKS-2 RNA

    Depositor

    Carmen Williams

    Article

    Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

    Insert

    Tankyrase 2 (TNKS2 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVC297 VEGF Site#1

    Plasmid

    #47505

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 1 (VEGFA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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