We narrowed to 26,401 results for: Vit

1. pCDH-CIBN-CAAX

   Plasmid

   #188989

   Purpose

   A lentiviral plasmid encoding plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   CIBN (CIB1 Mustard Weed)
   

   Tags

   CAAX

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-cofilin S3A

   Plasmid

   #163604

   Purpose

   Expresses constitutively active cofilin in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   cofilin1 (Cfl1 Mouse)
   

   Expression

   Mammalian

   Mutation

   S3A substitution to make cofilin constitutively a…

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSynapsin1-axon-GCaMP6m

   Plasmid

   #111261

   Purpose

   For enriched expression of GCaMP6m in axons

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV9

   Insert

   axon-GCaMP6m
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Promoter

   hSynapsin1

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SG3ΔENV CA Q63A,Q67A

   Plasmid

   #145784

   Purpose

   Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q63A,Q67A mutation in capsid (CA Q63A,Q67A).

   Depositor

   Wesley Sundquist

   Article

   Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

   Insert

   Capsid
   

   Use

   Lentiviral and Retroviral

   Expression

   Mammalian

   Mutation

   Changed Capsid residues Glutamines 63 and 67 to a…

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-Myo 1d

   Plasmid

   #134906

   Purpose

   Expression of rat Myo 1d (Myr 4) in mammalian cells.

   Depositor

   Martin Bähler

   Article

   Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

   Insert

   Myo 1d (rat) with 3' UTR (Myo1d Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

   Plasmid

   #220224

   Purpose

   AAV-STARR-seq validation control vector with Venus

   Depositor

   Simon Rumpel

   Article

   Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-FLAG-SpCas9-HF1

   Plasmid

   #126770

   Purpose

   Expression of increased fidelity SpCas9-HF1 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7-AsCas12a_crRNA-site4 (MSP3511)

   Plasmid

   #160139

   Purpose

   T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p944-SWITCH-OVER insert: EF1a-Puro

   Plasmid

   #217891

   Purpose

   insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag Nprl2 pLJM1

    Plasmid

    #46338

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Nprl2 (NPRL2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pFUGW-ZipACR-EYFP

    Plasmid

    #88844

    Purpose

    Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    ZipACR-NotI-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-Stra6L-6His

    Plasmid

    #174215

    Purpose

    Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

    Depositor

    Adrian Press

    Article

    Stafast et al ACS Appl. Polym. Mater. 2022

    Insert

    Stra6L (Stra6l Mouse)
    

    Tags

    6-His

    Expression

    Mammalian

    Mutation

    non

    Promoter

    CMV

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBabe-PI(WT)-Src(YF)-mCherry

    Plasmid

    #87357

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src isoform 2 (Src Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Y535F

    Promoter

    CMV

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eNme2-C TadCBEa

    Plasmid

    #193840

    Purpose

    Express TadCBEa (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    eNme2-C TadCBEa
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTRCHisB-TRF2∆M

    Plasmid

    #50489

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Delta Myb (TERF2 Human)
    

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 3-437

    Promoter

    pTrc

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNAIIIB cdc42 WT

    Plasmid

    #61635

    Purpose

    mammalian expression of cdc42 wt

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    cdc42 (CDC42 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

17. p2CT-His-MBP-Lbu_C2c2_R1079A

    Plasmid

    #83494

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R1079A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R1079A

    Promoter

    T7

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-BE4-RrA3F F130L

    Plasmid

    #138341

    Purpose

    Mammalian expression plasmid for BE4-RrA3F F130L

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-RrA3F F130L
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCW-CCND1-HA-puro

    Plasmid

    #212670

    Purpose

    Express tagged cyclin D1

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    TRE

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-H2B-mRuby3-IRES-eGtACR1-ST

    Plasmid

    #108960

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eGtACR1-ST
    

    Expression

    Mammalian

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge