We narrowed to 58,797 results for: Ran

1. pAS133

   Plasmid

   #211504

   Purpose

   AAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

   Depositor

   Randall Platt

   Article

   Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

   Insert

   mTagBF2
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-HA-C12orf66

   Plasmid

   #87048

   Purpose

   mammalian expression of C12orf66

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   C12orf66 (C12orf66 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP

   Plasmid

   #232754

   Purpose

   Expresses EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   EGFP
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEf1a OsTIR ires NEO pA T2BH

   Plasmid

   #127910

   Purpose

   Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   OsTIR
   

   Use

   Transposon

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMax_BFP_A191G

   Plasmid

   #177823

   Purpose

   Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   tagBFP_A191G
   

   Expression

   Mammalian

   Mutation

   A191G

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.2-DEST-MatryoshCaMP6s

   Plasmid

   #100025

   Purpose

   Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Tags

   V5 Epitope Tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N3-TPC2

   Plasmid

   #80153

   Purpose

   Intracellular Ca2+ store marker

   Depositor

   Santiago Di Pietro

   Article

   Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7

   Insert

   TPCN2 (TPCN2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

   Plasmid

   #130640

   Purpose

   Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6
   

   Use

   CRISPR; Transposon

   Tags

   His10-MBP-TEVsite on TniQ

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-cGAS-N-HA

   Plasmid

   #130913

   Purpose

   Expresses human cGAS N terminus(aa1-159)-HA; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   cGAS N terminus
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS132

    Plasmid

    #211503

    Purpose

    AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Insert

    mCherry
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBO.ACT006

    Plasmid

    #182716

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    GFP in cloning site of crRNA for easier screening…

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

    Plasmid

    #226011

    Purpose

    CasRx guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mRuby2-Tubulin-C-18

    Plasmid

    #55915

    Purpose

    Localization: Microtubules, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pRK5-HA-KPTN

    Plasmid

    #87042

    Purpose

    mammalian expression of KPTN

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    KPTN (KPTN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174883

    Purpose

    Targeting vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Pou5f1
    

    Use

    Mouse Targeting

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCLJ-F2P-LoxP-PGK-PuroR-LoxP

    Plasmid

    #227502

    Purpose

    Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    Prdm8 promoter
    

    Use

    AAV and Cre/Lox

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-ROS1-V5/HIS

    Plasmid

    #205609

    Purpose

    Expression of human ROS1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROS1 receptor tyrosine kinase (ROS1 Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CDK1as_T2A_Zeo

    Plasmid

    #118596

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. amilGFP chromoprotein

    Plasmid

    #117842

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilGFP
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-CMV-TetO2_3C-Avi-His6

    Plasmid

    #113887

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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