We narrowed to 10,961 results for: T7
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Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
Tags6xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
Tags8xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-S473A
Plasmid#49190PurposecDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalMutationS473APromoterT7Available SinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hBTC
Plasmid#199230PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N24_switch
Plasmid#132725PurposeT7 RNAP-driven expression of 3WJ repressor index 24 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N24_switch
ExpressionBacterialAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TGFB1
Plasmid#67080PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianPromoterCMVAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
Tags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dapp1-PH pET11a
Plasmid#71376PurposePhosphoinositide BindingDepositorInsertDapp1-PH (DAPP1 Human)
ExpressionBacterialMutationPleckstrin homology (PH) domain; aa 148-273PromoterT7Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
K401-iLid
Plasmid#122484PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-401 iLid His tag
TagsHis6 and iLidExpressionBacterialMutationKinesin amino acids 1-401PromoterT7Available SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFAM
Plasmid#67064PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU387
Plasmid#178981PurposepACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR arrayDepositorInsertCRISPR array from Candidatus Scalindua brodae
ExpressionBacterialPromoterT7Available SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
NpT7-5-ERK1
Plasmid#39229DepositorAvailable SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
ExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBT138b-pro1
Plasmid#138513PurposeP3hDepositorInsertT7-RNAP
UseSynthetic BiologyAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-PKCsub-YPet-NES
Plasmid#138207PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub-YPet-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Xyl-DocI
Plasmid#58711PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertXyl-DocI
TagsHIS, HRV 3C, and ybbRExpressionBacterialMutationchanged Threonin 120 to Cysteine in Xylanase Doma…PromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR(T/A)
Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorInsertsapphireAKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only