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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2

Plasmid

#197422

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

Plasmid

#197423

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pegRNA_HEK4_1bp-insertion

Plasmid

#201977

Purpose

Express a pegRNA used for 1bp insertion at HEK4 site

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

pegRNA_HEK4_1bp-insertion

Expression

Mammalian

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

Plasmid

#199254

Purpose

Expression construct encoding the high specificity SpCas9-KARA-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity S. pyogenes Cas9-D10A-KARA nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A R1060A

Promoter

CAG promoter

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

Plasmid

#199253

Purpose

Expression construct encoding the high specificity SpCas9-KA-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity S. pyogenes Cas9-D10A-KA nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mNudcd3 - 1

Plasmid

#198501

Purpose

lentiviral stable expression of mNudcd3 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mNudcd3 (Nudcd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mNudcd3 - 2

Plasmid

#198502

Purpose

lentiviral stable expression of mNudcd3 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mNudcd3 (Nudcd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mRab12-2

Plasmid

#198476

Purpose

lentiviral stable expression of mRab12 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse Rab12 (Rab12 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mRab12-1

Plasmid

#198475

Purpose

lentiviral stable expression of mRab12 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse Rab12 (Rab12 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNXF-1-E151A_O

Plasmid

#147118

Purpose

Mammalian Expression of HsNxf-1-E151A

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HsNxf-1-E151A (NXF1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNXF-1-R128ER158E_O

Plasmid

#147119

Purpose

Mammalian Expression of HsNxf-1-R128ER158E

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HsNxf-1-R128ER158E (NXF1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNXF-1-3xmut_O

Plasmid

#147115

Purpose

Mammalian Expression of HsNxf-1-3xmut

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HsNxf-1-3xmut (NXF1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pminiT-mEmerald-MED1 donor

Plasmid

#197867

Purpose

Donor plasmid used for building mEmerald-MED1 knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

CRSP210 (Med1 Mouse)

Use

Mouse Targeting

Tags

mEmerald

Promoter

NA

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTij_sg_mmu_Med6_C_terminal

Plasmid

#197869

Purpose

CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

Med6 (Med6 Mouse)

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

Plasmid

#192132

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-Y

Use

AAV

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (Y446A)

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-PB-SRT-tdTomato_BC11

Plasmid

#193174

Purpose

Barcoded piggyBac self-reporting transposon with tdTomato marker in AAV backbone

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac tdTomato SRT

Use

AAV

Mutation

Barcode: GAAG

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-PB-SRT-tdTomato_BC9

Plasmid

#193176

Purpose

Barcoded piggyBac self-reporting transposon with tdTomato marker in AAV backbone

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac tdTomato SRT

Use

AAV

Mutation

Barcode: TCGA

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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PB-SRT-Puro_BC4

Plasmid

#193146

Purpose

Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac Puro SRT

Expression

Mammalian

Mutation

Barcode: CAAC

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-PB-SRT-tdTomato_BC23

Plasmid

#193188

Purpose

Barcoded piggyBac self-reporting transposon with tdTomato marker in AAV backbone

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac tdTomato SRT

Use

AAV

Mutation

Barcode: CTAG

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-PB-SRT-tdTomato_BC22

Plasmid

#193187

Purpose

Barcoded piggyBac self-reporting transposon with tdTomato marker in AAV backbone

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac tdTomato SRT

Use

AAV

Mutation

Barcode: CAGT

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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