We narrowed to 58,539 results for: ran

1. pPBO.ACT006

   Plasmid

   #182716

   Purpose

   Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   CasRx crRNA cloning backbone
   

   Use

   CRISPR

   Tags

   GFP in cloning site of crRNA for easier screening…

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

   Plasmid

   #226011

   Purpose

   CasRx guide RNA cloning backbone (lenti)

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS132

   Plasmid

   #211503

   Purpose

   AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

   Depositor

   Randall Platt

   Article

   Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

   Insert

   mCherry
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mRuby2-Tubulin-C-18

   Plasmid

   #55915

   Purpose

   Localization: Microtubules, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Tubulin (TUBA1B Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174883

   Purpose

   Targeting vector for generating Pou5f1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Pou5f1
   

   Use

   Mouse Targeting

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-HA-KPTN

   Plasmid

   #87042

   Purpose

   mammalian expression of KPTN

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   KPTN (KPTN Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCLJ-F2P-LoxP-PGK-PuroR-LoxP

   Plasmid

   #227502

   Purpose

   Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   Prdm8 promoter
   

   Use

   AAV and Cre/Lox

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-ROS1-V5/HIS

   Plasmid

   #205609

   Purpose

   Expression of human ROS1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   ROS1 receptor tyrosine kinase (ROS1 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tol2-CAG::Nucbow

   Plasmid

   #158992

   Purpose

   Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Nucbow
   

   Tags

   All FPs are fused to H2B

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CDK1as_T2A_Zeo

    Plasmid

    #118596

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEYFP-hDF C2B

    Plasmid

    #72583

    Purpose

    human dysferlin C2B domain tagged with YFP

    Depositor

    Renzhi Han

    Article

    Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

    Insert

    dysferlin C2B domain (DYSF Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C2B domain

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

12. amilGFP chromoprotein

    Plasmid

    #117842

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilGFP
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-HA-ITFG2

    Plasmid

    #87045

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-CMV-TetO2_3C-Avi-His6

    Plasmid

    #113887

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC2-dual-dCas9VP48-sgExpression

    Plasmid

    #48236

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174882

    Purpose

    Targeting vector for generating Nanog STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Nanog
    

    Use

    Mouse Targeting

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. BtuF

    Plasmid

    #92209

    Purpose

    Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter

    Depositor

    Douglas Rees

    Article

    Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99

    Insert

    BtuF (btuF )
    

    Tags

    6x His Tag and PelB Signal Sequence

    Expression

    Bacterial

    Mutation

    BtuF signal sequence removed and uses the PelB si…

    Promoter

    T7 Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-CMV-TetO2_3C-mVenus-Twin-Strep

    Plasmid

    #113891

    Purpose

    Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-mVenus-Twin-Strep

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS L30 Ruby3-Rab6A

    Plasmid

    #135653

    Purpose

    Rab6A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

    Insert

    Rab6A
    

    Tags

    Ruby3

    Expression

    Mammalian

    Promoter

    L30

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. cjBlue chromoprotein

    Plasmid

    #117844

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, cjBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge