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We narrowed to 8,781 results for: construct

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Showing: 7061 - 7080 of 8781 results

  2. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid
    #199254
    Purpose
    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity S. pyogenes Cas9-D10A-KARA nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A R1060A
    Promoter
    CAG promoter
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199254-plasmid-map-sequence-id-391656

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  12. pEUK028

    Plasmid
    #180826
    Purpose
    T7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    SwGdmA (SWIT_RS16490 Sphingomonas wittichii RW1, Synthetic)
    Tags
    6X His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180826-plasmid-map-sequence-id-355555

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  13. pEUK037

    Plasmid
    #180829
    Purpose
    T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    CnGdmB (CNE_RS35790 Cupriavidus necator N-1)
    Tags
    6X His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180829-plasmid-map-sequence-id-355562

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  15. psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry

    Plasmid
    #182381
    Purpose
    Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters
    Depositor
    Chunghun Lim
    Article
    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1
    Inserts
    mtIF3 (Mtif3 Mouse)
    mCherry
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Changed CDS sequence from 'ccacgttcaagtcacga…
    Promoter
    HSV TK promoter and SV40 promoter
    Available Since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    182381-plasmid-map-sequence-id-358105

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  16. psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1

    Plasmid
    #182380
    Purpose
    Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters
    Depositor
    Chunghun Lim
    Article
    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1
    Inserts
    mtIF3 (Mtif3 Mouse)
    mito-mTFP1
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Changed CDS sequence from 'ccacgttcaagtcacga…
    Promoter
    CMV promoter and SV40 promoter
    Available Since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    182380-plasmid-map-sequence-id-358102

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  20. C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r

    Plasmid
    #162950
    Purpose
    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag
    Use
    Synthetic Biology
    Available Since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    162950-plasmid-map-sequence-id-331914

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Showing: 7061 - 7080 of 8781 results