We narrowed to 18,489 results for: Met

1. YEp195-CUP1-His-Ubi

   Plasmid

   #99540

   Purpose

   Yeast episomal vector for expression of His-tagged ubiquitin; URA3 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   UBI3(1-76) (RPL40A Budding Yeast)
   

   Tags

   7xHis

   Expression

   Yeast

   Promoter

   CUP1

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLH-sgRNA-Sirius-8XPP7

   Plasmid

   #121940

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-8XPP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   human U6 promoter

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKIKOrbsARCm

   Plasmid

   #46765

   Purpose

   E.coli genomic integration rbsAR locus

   Depositor

   Lars Nielsen, Claudia Vickers

   Article

   Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

   Type

   Empty backbone

   Use

   E.coli genomic integration

   Available Since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPL5606_TEF1*-Cre_ADE2

   Plasmid

   #60932

   Purpose

   Constitutive expression vector for Cre-recombinase containing ADE2 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Promoter

   mutant TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-Ec.coaD (pESC106)

   Plasmid

   #50388

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   PPAT
   

   Tags

   6xHis-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPR-v2 sgATG7

   Plasmid

   #211534

   Purpose

   Deletes ATG7

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   sgATG7
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-HisYFP-Sp100

   Plasmid

   #53141

   Purpose

   E.coli expression of HisYFP tagged human Sp100 fragment

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   Sp100A fragment (SP100 Human)
   

   Tags

   His-YFP

   Expression

   Bacterial

   Mutation

   fragment (amino acids 71-480); deletion of L75

   Promoter

   T7

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

   Plasmid

   #175534

   Purpose

   Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag7-myc_PDGFRtmb
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIS.MTRIA-NKB

   Plasmid

   #184609

   Purpose

   For expression of a fluorescent sensor for neurokinin B in mammalian cells

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-NKB
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIS.MTRIA-NPFF

    Plasmid

    #184612

    Purpose

    For expression of a fluorescent sensor for neuropeptide FF in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-NPFF
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKIKOrbsARKm

    Plasmid

    #46768

    Purpose

    E.coli genomic integration rbsAR locus

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

    Type

    Empty backbone

    Use

    E.coli genomic integration

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCGN R-Ras 43N

    Plasmid

    #14731

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Insert

    R-ras (RRAS Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    43N

    Available Since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

13. YEp181-CUP1-His-Ubi

    Plasmid

    #99539

    Purpose

    Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    UBI3(1-76) (RPL40A Budding Yeast)
    

    Tags

    7xHis

    Expression

    Yeast

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6p-1-BCCP-PupE

    Plasmid

    #113403

    Purpose

    Expresses BCCP-PupE in bacteria

    Depositor

    Min Zhuang

    Article

    Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

    Insert

    BCCP-PupE
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH47732:FCP:NAT

    Plasmid

    #85984

    Purpose

    Golden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    NAT
    

    Use

    Golden gate assembly

    Promoter

    FCP

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. scFv-GCN4-sfGFP-deadTET1CD

    Plasmid

    #184442

    Purpose

    Catalytically DEAD TET1CD with scFv against GCN4

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    Catalytically DEAD TET1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-CitronRH

    Plasmid

    #158545

    Purpose

    Mammalian expression of nonbinding variant of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitronRH
    

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPD555 ZF150x6-Compact_EYFP

    Plasmid

    #138869

    Purpose

    EYFP reporter vector for the ZF150x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF150x6-Compact
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    ZF150x6-Compact

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCGN R-Ras 38V

    Plasmid

    #14728

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Insert

    R-ras (RRAS Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    38V

    Available Since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pET28 Set9 wt

    Plasmid

    #24082

    Depositor

    Danny Reinberg

    Article

    Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

    Insert

    Set9 (SETD7 Human)
    

    Tags

    FLAG and His

    Expression

    Bacterial

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only