We narrowed to 10,515 results for: nar

1. pLX_HA-C7orf26v1

   Plasmid

   #178535

   Purpose

   Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_HA backbone

   Depositor

   William Hahn

   Article

   Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

   Insert

   C7orf26v1 (INTS15 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGB-As-NF-kB3

   Plasmid

   #180211

   Purpose

   Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB3 protein in yeast

   Depositor

   Thomas Gilmore

   Article

   Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

   Insert

   As-NF-kB3
   

   Use

   Tags

   GAL4 (aa 1-147) fusion protein

   Expression

   Yeast

   Mutation

   aa 2-412

   Promoter

   AdhI

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-FLAG-As-NF-kB3

   Plasmid

   #180202

   Purpose

   Expresses FLAG-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells

   Depositor

   Thomas Gilmore

   Article

   Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

   Insert

   As-NF-kB3
   

   Use

   Tags

   FLAG-tagged fusion protein

   Expression

   Mammalian

   Mutation

   aa 2-412

   Promoter

   CMV

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-FLAG-Co-NF-kB-Cterm

   Plasmid

   #180199

   Purpose

   Expresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cells

   Depositor

   Thomas Gilmore

   Article

   Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

   Insert

   Co-NF-kBCterm
   

   Use

   Tags

   FLAG-tagged fusion protein

   Expression

   Mammalian

   Mutation

   aa 542-1224

   Promoter

   CMV

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEos2-dVHH[GFP] in pCSDEST

   Plasmid

   #162870

   Purpose

   Super-resolution of GFP in culture

   Depositor

   Rob Parton

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   mEos2-dVHH[EGFP]
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. deltaLoop-MIDAS

   Plasmid

   #171605

   Purpose

   Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

   Insert

   MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
   

   Use

   Tags

   His6 tag and SNAP tag

   Expression

   Bacterial

   Mutation

   Replaced amino acids 4458-4496 with ASGSGS linker

   Promoter

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hy_pMT Flag-URH49 MUT

   Plasmid

   #110126

   Purpose

   Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   URH49 (DDX39A Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 amino acid mutations (RSFS motif changed to KSL…

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hy_pMT Flag-Hel25E MUT

   Plasmid

   #110122

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 mutations (KKLN motif changed to RSFS)

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)

   Plasmid

   #153439

   Purpose

   E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSR357.12

    Plasmid

    #125107

    Purpose

    Expression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)134
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    A84T - see depositor comments below and Q183K-see…

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCu-GFP-Atg17(416) (L313D I314K)

    Plasmid

    #121409

    Purpose

    Expressing GFP-Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-GFP-ATG17 ORF-CYC1 terminator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 313 to Aspartic acid; Isoleucine 314 to …

    Promoter

    CUP1 promoter

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

    Plasmid

    #104619

    Purpose

    Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. RSV/mGATA3 (antisense)

    Plasmid

    #83817

    Purpose

    contains antisense mouse GATA3

    Depositor

    Douglas Engel

    Article

    Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

    Insert

    GATA3-antisense strand (Gata3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pMKO.1-puro/hFOXH1 shRNA6

    Plasmid

    #59310

    Purpose

    Knockdown of human FOXH1

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    hFOXH1 shRNA (FOXH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

    Plasmid

    #188768

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

    Plasmid

    #188777

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-Flag-c-Myc

    Plasmid

    #102626

    Purpose

    Expresses c-Myc in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    MYC proto-oncogene, bHLH transcription factor (MYC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-26b-NbVHH05-HA-His

    Plasmid

    #171567

    Purpose

    Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-HA-His (UBE2J1 Human)
    

    Use

    Tags

    HA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-CAAX

    Plasmid

    #86056

    Purpose

    A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Homo sapiens Harvey rat sarcoma (HRAS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains the region from 825-857 of HRAS as a far…

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188766

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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