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pHAGE-EFS-N22P-sfGFP-NLS

Plasmid

#236389

Purpose

Overexpression of N22P-3xEGFP in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

N22P-3xEGFP

Use

Lentiviral

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pATDt

Plasmid

#249499

Purpose

Expresses tdTomato – Monodirectional chloroplast expression vector (psbH Rescue) in Chlamydomonas Reinhardtii photosynthetically deficient strain CC4388

Depositor

Isabel Desgagné-Penix

Insert

tdTomato (red fluorescence)

Use

Chlamydomonas reinhardtii chloroplast genome

Expression

Bacterial

Mutation

tdTomato results from the fusion of two copies of…

Promoter

The psbD/ 5′ UTR, a light-activated chloroplast p…

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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LNPL1093-piggybac-Dox-inducible-CasRx-QK634-UnirapR-IRES-mCherry

Plasmid

#248111

Purpose

Expresses dox-inducible ChemoCas13d or CasRx-QK634-LightR (UnirapR domain inserted right after amino acid residue 634 of CasRx)

Depositor

Jared Toettcher

Article

Zhu et al bioRxiv 2025.04.21.649818

Insert

CasRx-UnirapR

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

Tet inducible

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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LNPL503-piggybac-Dox-inducible-CasRx-QK634-LightR-IRES-mCherry

Plasmid

#248105

Purpose

Expresses dox-inducible OptoCas13d or CasRx-QK634-LightR (LightR domain inserted right after amino acid residue 634 of CasRx)

Depositor

Jared Toettcher

Article

Zhu et al bioRxiv 2025.04.21.649818

Insert

CasRx-LightR

Use

CRISPR and Synthetic Biology

Promoter

Tet inducible

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn1-mCherry-CAAX

Plasmid

#223674

Purpose

AAV expression of mCherry from hSyn1 promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mCherry-CAAX

Use

AAV

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAMKII-DrBphP-PCM

Plasmid

#241775

Purpose

To produce AAV9 encoding CAMKII-DrBphP-PCM

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2025 Jul 14;16(1):6469. doi:

Insert

DrBphP-PCM

Use

AAV

Promoter

CAMKII

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT7R-7xsfGFP11-HA-HDR-donor

Plasmid

#221818

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT7R coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT7R homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT7R-sfGFP11-HA-CRISPR-HDR-donor

Plasmid

#221819

Purpose

Donor plasmid for inserting GFP11-HA to the end of Drosophila 5-HT7R coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

GFP11-HA donor with homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor

Plasmid

#221832

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1AR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2BR-7xsfGFP11-HA-HDR-donor

Plasmid

#221833

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2BR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_2xFlag-dSERT-HDR-donor-HDR

Plasmid

#221835

Purpose

Donor plasmid for inserting 2xFLAG at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

2xFLAG with dSERT homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT1BR-7xsfGFP11-HA-HDR-donor

Plasmid

#221837

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1BR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

Plasmid

#221838

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2AR(isoformBFH)-7xsfGFP11-HA-HDR-donor

Plasmid

#221839

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoforms BFH) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pACUH 5-HT2BR-7xGFP11-HA

Plasmid

#221840

Purpose

Plasmid for generating 10xUAS-5-HT2BR-7xGFP11-HA transgenic flies with attP/attB integration. It carries the attB sequence.

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

5-HT2BR (CG42796 Fly)

Tags

7xGFP11-HA

Expression

Insect

Promoter

hsp70

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRuby2-WPRE

Plasmid

#240051

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

mRuby2

Use

AAV

Promoter

CAG

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-MCP-3XBFP-NLS

Plasmid

#236372

Purpose

Overexpression of MCP-3XBFPnls in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

MCP-3XBFPnls

Use

Lentiviral

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-ASAP3-WPRE

Plasmid

#240052

Purpose

Constitutive expression of genetically encoded voltage indicator ASAP3

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

ASAP3

Use

AAV

Promoter

CaMKII

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Ace-mNeon1-WPRE

Plasmid

#240053

Purpose

Constitutive expression of genetically encoded voltage indicator Ace-mNeon1

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

Ace-mNeon1

Use

AAV

Promoter

CaMKII

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_-DIO-Varnam1-WPRE

Plasmid

#240054

Purpose

Cre dependent Red fluorescent voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

VARNAM1

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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