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p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

Plasmid

#224536

Purpose

A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-FLAG- NLS-mScarlet-I3

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ER-mRFP

Plasmid

#231176

Purpose

Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, in mammalian cells

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

ER-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-mTurquoise-P2A-iCre (JDW 920)

Plasmid

#224518

Purpose

A Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mTurquoise-P2A-iCre

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC6A6

Plasmid

#132196

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A6 (SLC6A6 Human)

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-FastFUCCI (JDW 1488)

Plasmid

#242556

Purpose

Gateway middle entry clone containing FastFUCCI reporter; For visualizing cell cycle state

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC9A2

Plasmid

#132222

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9A2 (SLC9A2 Human)

Expression

Mammalian

Available Since

Nov. 7, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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p3E-WPRE-SV40-pA (JDW 922)

Plasmid

#224545

Purpose

A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

WPRE-SV40-pA

Use

Gateway cloning

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC38A8

Plasmid

#132082

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A8 (SLC38A8 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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p2xFlag CMV2-YAP2-1st WW mutant

Plasmid

#19046

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein isoform 3 (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine,and Proline 202…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2-YAP2-2nd WW mutant

Plasmid

#19047

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 258 changed to Alanine, and Proline 2…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC30A3

Plasmid

#132101

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A3 (SLC30A3 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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8xNFAT-ZsG-muhCD4

Plasmid

#162745

Purpose

NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene

Depositor

Maki Nakayama

Article

Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Human CD4 gene with glutamine to tyrosine at posi…

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A26

Plasmid

#132259

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A26 (SLC25A26 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC2A2

Plasmid

#132126

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A2 (SLC2A2 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC5A2

Plasmid

#132241

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC5A2 (SLC5A2 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC6A14

Plasmid

#131865

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A14 (SLC6A14 Human)

Expression

Mammalian

Available Since

Oct. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221 UBR5 ∆UBR

Plasmid

#81063

Purpose

Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)

Use

Cloning

Mutation

W1235L (bp g3704t)

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pME-3xNLS-mTagBFP2-Cre (JDW 918)

Plasmid

#224517

Purpose

A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xNLS-mTagBFP2-Cre

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC38A9

Plasmid

#132070

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A9 (SLC38A9 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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