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Showing: 7141 - 7160 of 23124 results

  1. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid
    #177939
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D614G, N501Y
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177939-plasmid-map-sequence-id-348111

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  2. pHSN501

    Plasmid
    #50589
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50589-plasmid-map-sequence-id-75662

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  6. YFVdelNS1-mVenus

    Plasmid
    #179950
    Purpose
    pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with mVenus
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    YFV-17D lacking Nonstructural protein 1 (NS1) but with mVenus
    Use
    Transneuronal tracing
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    179950-plasmid-map-sequence-id-357475

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  10. YFVdelNS1-Cre

    Plasmid
    #179952
    Purpose
    pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with Cre
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    YFV-17D lacking nonstructural protein 1 (NS1), but with Cre
    Use
    Transneuronal tracing
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Available since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    179952-plasmid-map-sequence-id-354333

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  11. GABA(A) receptor subunit B3SE

    Plasmid
    #49171
    Purpose
    pHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.
    Insert
    GABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
    Use
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Mutation
    GTG (V) to TGC (C)
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49171-plasmid-map-sequence-id-72502

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  16. pLJM1-Flag-Rheb-4Rs

    Plasmid
    #165022
    Purpose
    expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)
    Depositor
    Ken Inoki
    Article
    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d
    Insert
    Rheb-4Rs (RHEB Human)
    Use
    Lentiviral
    Tags
    flag
    Expression
    Mammalian
    Mutation
    change lysine residues on position 109, 135, 151,…
    Promoter
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165022-plasmid-map-sequence-id-326717

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  20. TC1316

    Plasmid
    #164883
    Purpose
    CMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3
    Depositor
    Tian Chi
    Article
    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:
    Insert
    dcas13d-ADARdd
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dCas13d L560-G586 deleted
    Promoter
    CMV, T7
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164883-plasmid-map-sequence-id-325957

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Showing: 7141 - 7160 of 23124 results