We narrowed to 17,934 results for: multi
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Plasmid#40386DepositorInsertsUseBaculovirusTags6xHis and HisTEVMutationWT aa1867-2186PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits
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pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4
Plasmid#214137PurposeInsect cell expression of the human 20SCP proteasome assembly chaperonesDepositorInsertsExpressionInsectPromoterpolyhedrinAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TK4_SH7_targeting
Plasmid#254454PurposeTK4 targeting vector containing left and right homology arms for the SH7 locusDepositorInsertsmycNLS-mScarlet-T2A-mNeonGreen-PEST
puroR-T2A-mycNLS-mTagBFP2
UsePiggybacTagsPEST, T2A-mycNLS-mTagBFP2, and c-myc-NLSExpressionMammalianPromoterCAG and EF1aAvailable sinceApril 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
cBEST4
Plasmid#234660PurposeExpresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertionDepositorInsertsspCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP3 and tcp830Available sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDIV270
Plasmid#226181PurposeExpresses Cas9 and gRNA targeting KU70 gene in K. phaffii.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRExpressionBacterial and YeastPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQ-NFY-A-GB-HC
Plasmid#171081PurposeCo-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYCDepositorTagsGST and HisExpressionBacterialPromoterT5 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
GRM1-DuET
Plasmid#213300PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SARS-CoV-2-Nsp3PLpro
Plasmid#204208PurposeMammalian expression of SARS-CoV-2 Nsp3DepositorInsertSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
TagsFLAGx1ExpressionMammalianMutationPLpro of Nsp3Available sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
AWP-029
Plasmid#213966PurposeExpresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.DepositorInsertCas12a (cas12a Segatella bryantii, Synthetic)
Tags3XFLAGExpressionBacterialMutationD875A to inactivate target nuclease activityPromoterPcfxA, IPTG inducibleAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Pink Flamindo-p2a-bPAC
Plasmid#202716PurposeMammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclaseDepositorPublicationInsertsPink Flamindo
bPAC
UseLentiviralTags10xHis and mycExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-VARNAM2-Kv2.1PR
Plasmid#195533PurposeCre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxExpressionMammalianMutationVARNAM SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
rTetR-GFP-VP16
Plasmid#183917PurposeVP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline additionDepositorAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2
Plasmid#178574PurposeLentiviral vector for CamKIIα::Opto-vTrapDepositorInsertCRY2PHR-P2A-CIBN-mCherry-VAMP2
UseLentiviralPromoterCaMKIIaAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDAS12342_PEAR-GFP_2in1
Plasmid#177184PurposePEAR-GFP plasmid with a pegRNA that targets its own plasmidDepositorInsertsEGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid
UseCRISPRExpressionMammalianMutationdisrupted 5' splice sitePromoterCMV and U6Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSN075
Plasmid#166732PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS2-Spike-D614G
Plasmid#166850PurposeFor expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)DepositorAvailable sinceJune 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
p006-GFP-Logic-AND
Plasmid#112237PurposeGFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.DepositorPublicationInsertsGFP under control of doubly-repressible promoter
GFP
araC
ExpressionBacterialPromoterAND-gate and pLacIAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (p15A origin)
Plasmid#74089PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorPublicationInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEL12
Plasmid#107918Purposehph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEM-Cas9HF1-indRecA56
Plasmid#102294PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
UseCRISPRExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only