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pET28-NFMshuf2

Plasmid

#232059

Purpose

Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2

Depositor

Sanjay Kumar

Article

Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

Neurofilament-Medium tail domain, charge shuffle 2

Tags

6xHis, thrombin

Expression

Bacterial

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP DEPP1-mCherry

Plasmid

#224559

Purpose

Expresses DEPP1 fused to mCherry in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

DEPP1 (DEPP1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP DEPP1 dTSnare-mCherry

Plasmid

#224561

Purpose

Expresses DEPP1 dTsnare fused to mCherry in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

DEPP1 (DEPP1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

removed Tsnare domain

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Plasmid

#205761

Purpose

Single-color FUCCI4 sensor for G1/S cell cycle transition and M/G1 transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL1027

Plasmid

#225598

Purpose

The STU backbone vector for rice genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL1028

Plasmid

#225599

Purpose

The STU backbone vector for maize genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-Rab5

Plasmid

#228139

Purpose

Expresses GFP-Rab5 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Rab5 (Rab5a Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-SNAP-Omp25

Plasmid

#228137

Purpose

Expresses SNAP-Omp25 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Mutation

transmembrane region (C-terminus)

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAM221

Plasmid

#226645

Purpose

pET24b-p97-R191Q-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His

Expression

Bacterial

Mutation

R191Q

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM224

Plasmid

#226647

Purpose

pET24b-p97-T262A-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His

Expression

Bacterial

Mutation

T262A

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAM220

Plasmid

#226644

Purpose

pET24b-p97-R159H-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His

Expression

Bacterial

Mutation

R159H

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAM219

Plasmid

#226643

Purpose

pET24b-p97-R155H-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His

Expression

Bacterial

Mutation

R155H

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAM229

Plasmid

#226651

Purpose

ybbR-p97-R155H-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His and ybbR

Expression

Bacterial

Mutation

R155H

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAM231

Plasmid

#226653

Purpose

ybbR-p97-T262A-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His and ybbR

Expression

Bacterial

Mutation

T262A

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAM222

Plasmid

#226646

Purpose

pET24b-p97-L198W-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His

Expression

Bacterial

Mutation

L198W

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAM234

Plasmid

#226656

Purpose

pcDNA3.1-p97-A232E-FLAG

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

FLAG

Expression

Mammalian

Mutation

A232E

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-KaNLuc

Plasmid

#208855

Purpose

Expresses FLAG epitope-tagged KaNLuc "Red" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

KaNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEY142

Plasmid

#223489

Purpose

unc-17(3k) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-17(3k) (unc-17 Nematode)

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY136

Plasmid

#223483

Purpose

tag-168 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tag-168

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY120

Plasmid

#223467

Purpose

sra-11(pB) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

sra-11(pB) (sra-11 Nematode)

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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