162,325 results

1. pCW57.1 Lysosomal-METRIQ

   Plasmid

   #135401

   Purpose

   Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

   Depositor

   Eisuke Itakura

   Article

   Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

   Insert

   DNAse II alpha (DNASE2 Human)
   

   Use

   Lentiviral

   Tags

   T2A, mCherry, and sfGFP

   Expression

   Mammalian

   Mutation

   Single-nucleotide polymorphism C432T

   Promoter

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2004

   Plasmid

   #143330

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXD1 (FOXD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV-RFP

   Plasmid

   #26001

   Depositor

   Elaine Fuchs

   Article

   Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

   Insert

   H2B-RFP (H2BC21 Human, A. victoria)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

   Plasmid

   #63166

   Purpose

   GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-HA"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_CALR sgRNA / hSpCas9

   Plasmid

   #172838

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pES005 (pTet-qacR wt/pQacA-deGFP)

   Plasmid

   #69034

   Purpose

   reporter with wild-type qacR on single plasmid

   Depositor

   Richard Murray

   Article

   de Los Santos et al ACS Synth Biol. 2015 Aug 19.

   Inserts

   qacR (qacR Synthetic)
   deGFP
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   pQacA and pTet

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-SEAP

   Plasmid

   #24595

   Depositor

   Alan Cochrane

   Insert

   SEAP (ALPP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781)

   Plasmid

   #199777

   Purpose

   enhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Has Service

   AAV PHP.eB

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   R297T for reduced recombinase efficiency.

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CamKIIa-jGCaMP8m-WPRE

   Plasmid

   #176751

   Purpose

   AAV-mediated expression of GCaMP8m under the CamKIIa promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CamKIIalpha

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBad-CA

    Plasmid

    #105665

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1

    Plasmid

    #212522

    Purpose

    Encodes SARS-CoV-2 variant XBB.1 Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike XBB.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBad33_ISAam1_reRNA

    Plasmid

    #205322

    Purpose

    Bacterial expression of ISAam1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISAam1 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. RPO41

    Plasmid

    #205072

    Purpose

    RPO41 protein expression

    Depositor

    Smita Patel

    Article

    Tang et al J Biol Chem. 2009 Feb 27;284(9):5514-22.

    Insert

    RPO41 (RPO41 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks N Terminal 100 amino acids

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4 myc PGC-1 alpha

    Plasmid

    #10974

    Purpose

    Mammalian expression of PGC-1a with myc and his tags

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

    Insert

    PGC-1 alpha (PPARGC1A Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-CMV-Galpha-q ONE-GO

    Plasmid

    #189738

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-q ONE-GO
    GNAQ (Gnaq Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. IF-GFP-ATRX

    Plasmid

    #45444

    Purpose

    ATRX expression vector

    Depositor

    Michael Dyer

    Article

    Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

    Insert

    ATRX (ATRX Mouse, Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Bacterial and Mammalian

    Mutation

    isoform 2, missing codon E124

    Promoter

    PCMV

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMU-PERr

    Plasmid

    #61202

    Purpose

    Fluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    mCherry-SKL
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. nesprin TSmod 25

    Plasmid

    #125555

    Purpose

    Nesprin-2G force sensor with cerulean-F25-venus TSmodule

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    mini-nesprin-2G (Syne2 Mouse)
    

    Use

    Tags

    cerulean, venus

    Expression

    Mammalian

    Mutation

    TSmod changed from mTFP to cerulean, flageliform …

    Promoter

    CMV

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNPTS138 Cm

    Plasmid

    #41891

    Depositor

    Howard Steinman

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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