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  4. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    U1a promoter
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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  7. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid
    #218710
    Purpose
    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218710-plasmid-map-sequence-id-433357

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  11. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

    Plasmid
    #160621
    Purpose
    Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold
    Depositor
    Diego Orzaez
    Article
    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-
    Insert
    U6-26:gRNA 4 (pNos): 2.1 aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U6-26
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160621-plasmid-map-sequence-id-322006

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  15. pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

    Plasmid
    #199263
    Purpose
    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    U1a
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199263-plasmid-map-sequence-id-389813

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  18. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

    Plasmid
    #221448
    Purpose
    Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3
    Depositor
    Omar Akbari
    Inserts
    wtCas9-NG
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221448-plasmid-map-sequence-id-437819

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Showing: 701 - 720 of 4687 results