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pAAV-Ef1a-DIO-OMOR-eYFP

Plasmid

#66847

Purpose

Expresses Opto-MOR in a Cre dependent manner

Depositor

Michael Bruchas

Article

Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

Insert

Opto Mu opioid receptor

Use

AAV and Cre/Lox

Tags

eYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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YFP-DBD

Plasmid

#14874

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

DAG binding domain (Prkcb Rat)

Tags

YFP

Expression

Mammalian

Mutation

Y123W

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-CIB1-mRFP1-MP

Plasmid

#58367

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Tags

mRFP1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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CD200RCD4d3+4-bio-linker-His

Plasmid

#36153

Purpose

A positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmid

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200R (Cd200r1 Rat)

Tags

His tag, biotinylation peptide, and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2012

Availability

Academic Institutions and Nonprofits only

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pEF Synaptophysin-GCamp6s

Plasmid

#188981

Purpose

Presynaptic fluorescent Ca2+ indicator

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Synaptophysin (Syp Synthetic, Rat)

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFsynW SYB2 reporter

Plasmid

#197276

Purpose

Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYB2 and HaloTag (Vamp2 Rat)

Use

Lentiviral

Tags

FLAG

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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mMaple-OMP25

Plasmid

#141151

Purpose

Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat)

Tags

mMaple

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP3bright

Plasmid

#74423

Purpose

Express fluorescent calcium indicator GCaMP3bright in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

Insert

GCaMP3bright

Expression

Mammalian

Mutation

D392A

Promoter

CMV

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_rSERT_WT

Plasmid

#190743

Purpose

For expression of wild-type rat SERT in mammalian cells

Depositor

Gary Rudnick

Article

Singh et al bioRxiv 2022.06.13.495991

Insert

SLC6A4 Serotonin Transporter (Slc6a4 Rat)

Tags

FLAG, His6, and c-myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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CMV::ratSyGCaMP2

Plasmid

#26124

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (Syp Rat)

Expression

Mammalian

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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Erk2

Plasmid

#29582

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Erk2 (Mapk1 Rat)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Starting Met removed from Erk2

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

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mCherry-RatLC3B-C-7

Plasmid

#55128

Purpose

Localization: Microtubule-Binding Protein, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

LC3B

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pORANGE Gria3-GFP KI

Plasmid

#131491

Purpose

Endogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria3, Gria3 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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C1-F-tractin-mCitrine

Plasmid

#155217

Purpose

Monitor global F-actin distribution

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) rat C/EBP alpha

Plasmid

#12550

Depositor

Peter Johnson

Article

Miller et al J Biol Chem. 2003 Apr 25;278(17):15178-8

Insert

CCAAT/Enhancer-binding Protein alpha (Cebpa Rat)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DNM2-R465W

Plasmid

#155262

Purpose

Mammalian expression of rat DNM2 fused to HA

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

dynamin 2 (Dnm2 Rat)

Tags

HA

Expression

Mammalian

Mutation

Arginine 465 to Tryptophan

Available Since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-VGF

Plasmid

#107575

Purpose

Encodes the entire VGF cDNA sequence under the control of CMV promoter. Allows expression of VGF in mammalian cells.

Depositor

Andrea Levi

Insert

VGF (Vgf Rat)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLIC-myc-GBAi W211A

Plasmid

#171754

Purpose

For the mammalian expression of myc-GBAi W211A

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

myc

Expression

Mammalian

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA

Plasmid

#174900

Purpose

amber suppression mediated CRFR1 A95TAG expression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Front Chem. 2021 Nov 11;9:768535. doi: 1

Insert

CRFR1 (Crhr1 Rat)

Tags

HA

Expression

Mammalian

Mutation

A95TAG

Promoter

EF1

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Bsn KI

Plasmid

#139664

Purpose

Endogenous tagging of Bassoon: N-terminal (amino acid position: L7)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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