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Showing: 1401 - 1440 of 2070 results

  1. pLEX307_CR-PDHA2S293A

    Plasmid
    #115204
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115204-plasmid-map-sequence-id-231794

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  2. pLEX307_CR-PDHA2

    Plasmid
    #115199
    Purpose
    Lentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115199-plasmid-map-sequence-id-232425

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  3. pLEX307_CR-PDHA2S291A

    Plasmid
    #115203
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115203-plasmid-map-sequence-id-232429

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  4. AAV-KrasG13S/sgKras/Cre

    Plasmid
    #99859
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    99859-plasmid-map-sequence-id-200267

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  5. AAV-KrasG13A/sgKras/Cre

    Plasmid
    #99855
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    99855-plasmid-map-sequence-id-200262

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  6. AAV-KrasG13R/sgKras/Cre

    Plasmid
    #99858
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99858-plasmid-map-sequence-id-193748

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  7. pLEX307_CR-PDHA2S291A/S293A

    Plasmid
    #115205
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A/S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291A/S293A (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115205-plasmid-map-sequence-id-232427

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  8. pPS808

    Plasmid
    #8856
    Depositor
    Pamela Silver
    Type
    Empty backbone
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 3, 2005
    Availability
    Academic Institutions and Nonprofits only

  9. pPS809

    Plasmid
    #8935
    Depositor
    Pamela Silver
    Type
    Empty backbone
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 19, 2005
    Availability
    Academic Institutions and Nonprofits only

  10. pPS810

    Plasmid
    #8936
    Depositor
    Pamela Silver
    Type
    Empty backbone
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 19, 2005
    Availability
    Academic Institutions and Nonprofits only

  11. pLD-puro-Cc-CR-PARK7WT-VA

    Plasmid
    #115187
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Expression
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115187-plasmid-map-sequence-id-225414

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  12. pLD-puro-Cc-CR-PARK7V51G-VA

    Plasmid
    #115188
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Expression
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115188-plasmid-map-sequence-id-225424

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  13. pLD-puro-Cc-CR-PARK7C53A-VA

    Plasmid
    #115189
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Expression
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115189-plasmid-map-sequence-id-225429

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  14. pLD-puro-Cc-CR-PARK7H126A-VA

    Plasmid
    #115190
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Expression
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115190-plasmid-map-sequence-id-225431

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  15. pLD-puro-Cc-CR-PARK7E163K-VA

    Plasmid
    #115191
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Expression
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115191-plasmid-map-sequence-id-225432

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  16. pPS293

    Plasmid
    #8851
    Depositor
    Pamela Silver
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 3, 2005
    Availability
    Academic Institutions and Nonprofits only

  17. p35S:TN3-YFPn

    Plasmid
    #102407
    Purpose
    split YFP. Plant expression of p35S:TN3-YFPn
    Depositor
    Silke Robatzek
    Insert
    TN3
    Use
    Tags
    YFPn
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    102407-plasmid-map-sequence-id-197775

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  18. pcDNA3.1-hDAT

    Plasmid
    #32810
    Depositor
    Susan Amara
    Insert
    DAT (SLC6A3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    32810-plasmid-map-sequence-id-445138

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  19. OTV-dDAT

    Plasmid
    #32812
    Depositor
    Susan Amara
    Insert
    dDAT (DAT Fly)
    Use
    Oocyte expression
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. R777-E269 Hs.RASSF9

    Plasmid
    #70553
    Purpose
    Gateway ORF clone of human RASSF9 [NM_005447.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASSF9 (RASSF9 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70553-plasmid-map-sequence-id-126002

    Enlarge

  21. R777-E270 Hs.RASSF9-nostop

    Plasmid
    #70554
    Purpose
    Gateway ORF clone of human RASSF9 [NM_005447.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASSF9 (RASSF9 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70554-plasmid-map-sequence-id-126003

    Enlarge

  23. p35S::ARA6/RabF1-YFPc

    Plasmid
    #102408
    Purpose
    split YFP. Plant expression of p35S::ARA6/RabF1-YFPc
    Depositor
    Silke Robatzek
    Insert
    ARA6 (ARA6 Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102408-plasmid-map-sequence-id-197788

    Enlarge

  24. pAcGP67A - murine TfR

    Plasmid
    #12392
    Depositor
    Pamela Bjorkman
    Insert
    transferrin receptor (Tfrc Mouse)
    Use
    Tags
    6X-His tag, Factor Xa cleavage site, and leader p…
    Expression
    Insect
    Mutation
    See comments section
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    12392-plasmid-map-sequence-id-274275

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  26. 35S:YFPc-RabG3b-DN

    Plasmid
    #102374
    Purpose
    fluorescent tagging. Plant expression of YFPc-RabG3b-DN
    Depositor
    Silke Robatzek
    Insert
    RabG3B (RABG3B Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    T22N
    Promoter
    35S
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    102374-plasmid-map-sequence-id-197538

    Enlarge

  27. 35S:YFPc-RabG3b-WT

    Plasmid
    #102373
    Purpose
    fluorescent tagging. Plant expression of YFPc-RabG3b-WT
    Depositor
    Silke Robatzek
    Insert
    RabG3B (RABG3B Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    102373-plasmid-map-sequence-id-197537

    Enlarge

  31. 35S:YFPc-RabG3b-CA

    Plasmid
    #102372
    Purpose
    fluorescent tagging. Plant expression of YFPc-RabG3b-CA
    Depositor
    Silke Robatzek
    Insert
    RabG3B (RABG3B Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    Q67L
    Promoter
    35S
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    102372-plasmid-map-sequence-id-197536

    Enlarge

  32. DRD2-3xHA/pCDNA3.1

    Plasmid
    #196940
    Purpose
    Plasmid expressing an antigen targeting the D2 dopamine receptor
    Depositor
    James Trimmer
    Insert
    DRD2 (DRD2 Human)
    Use
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196940-plasmid-map-sequence-id-387879

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Showing: 1401 - 1440 of 2070 results