We narrowed to 1,873 results for: FRT

1. pcDNA5/FRT/TO HIS DNAJB8

   Plasmid

   #19558

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT/TO-Hygro-3xFLAG

   Plasmid

   #185359

   Purpose

   Vector backbone for mammalian expression of proteins with an N-terminal 3xFLAG tag.

   Depositor

   Ayaz Najafov

   Insert

   3xFLAG
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-T0-Flag-HROB

   Plasmid

   #135298

   Purpose

   Expresses Flag-tagged HROB in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

   Insert

   HROB (HROB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCDNA5/FRT_CoA-Snifit(G41)

   Plasmid

   #197945

   Purpose

   Expression of CoA-Snifit in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

   Insert

   Halo-GFP-ecPanK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PanK=PanK-D127A,L277W,F252Y

   Promoter

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT_CoA-Snifit(V97T)

   Plasmid

   #197947

   Purpose

   Expression of CoA-Snifit in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

   Insert

   Halo-GFP-ecPanK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PanK=PanK-V97T, D127A,L277W,F252Y

   Promoter

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5 FRT TO GFP MPS1 K-R

   Plasmid

   #59819

   Purpose

   Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

   Insert

   Mps1 (TTK Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   KD, catalytically inactive, D664A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT-TO-hPKAalpha-Flag

   Plasmid

   #185380

   Purpose

   For mammalian expression of human PKAalpha-FLAG

   Depositor

   Ayaz Najafov

   Insert

   PRKACA (PRKACA Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHD-mW-attPCC-FRT (pTL717)

   Plasmid

   #115158

   Purpose

   Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.

   Depositor

   Tobias Langenhan

   Article

   Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

   Insert

   mW attP-CC FRT
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO HIS HSPA6

   Plasmid

   #19540

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 6 (HSPA6 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5/FRT/TO HIS HSPA14

    Plasmid

    #19542

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 14 (HSPA14 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

    Plasmid

    #157701

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA5/FRT/TO HIS DNAJA3

    Plasmid

    #19547

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEXfrt-SaCas9-U6-sgGabrg1

    Plasmid

    #124870

    Purpose

    Mutagenesis of Gabrg1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg1 (Gabrg1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT/TO/HIS/FLAG/HA-API5

    Plasmid

    #38074

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    API5 apoptosis inhibitor 5 (API5 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST(frt)(ATG_V5-APEX2_noSTOP)

    Plasmid

    #168207

    Purpose

    Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection

    Depositor

    Markus Hafner

    Article

    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

    Type

    Empty backbone

    Use

    Unspecified; Pdest gateway

    Tags

    V5 APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT/Flag-hINO80N2 (1-406)

    Plasmid

    #29442

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    INO80 (INO80 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    INO80 1-406 (a.a.)

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pFRT/TO/GFP-SCAF8 (CRISPR_resistant)

    Plasmid

    #122472

    Purpose

    Dox-inducible GFP (N-terminal tag) SCAF8 expression

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF8 (SCAF8 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    CMV

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO HIS SSA1

    Plasmid

    #19543

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    SSA1 (SSA1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H

    Plasmid

    #108376

    Purpose

    FRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5/FRT-ChemoB-NAD

    Plasmid

    #193824

    Purpose

    CMV overexpression of ChemoB-NAD in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoB-NAD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EBFP2:N39Y, A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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