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pMAPLe4 PCMTD1 T62 H67 M68

Plasmid

#246506

Purpose

Recombinant protein expression of PCMTD1 T62 H67 M68

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Residue 312 is Isoleucine in this plasmid but occ…

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eGFP-VSV

Plasmid

#242781

Purpose

AAV transfer plasmid expressing eGFP-VSV under a CAG promoter.

Depositor

Andrew Payne

Article

Park et al bioRxiv 2025.09.26.678648

Insert

EGFP

Use

AAV

Tags

VSV G tag

Promoter

CAG

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-hhN-HA

Plasmid

#240236

Purpose

Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

hedgehog N-terminal fragment (hh Fly)

Tags

HA-spGFP11

Expression

Insect

Mutation

aa 1-257

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_hhN-HA

Plasmid

#240225

Purpose

Gateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

hedgehog N-terminal fragment (hh Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Mutation

aa 1-257

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 AUP1 61-410

Plasmid

#185334

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 61-610 only

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-gp78 RING

Plasmid

#185353

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 cytoplasmic domain (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

Stop codon after aa 393

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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ERAP1-rs27895(T)

Plasmid

#206143

Purpose

Expresses the rs27895-T allele of ERAP1, myc-tagged.

Depositor

Dennis Ko

Article

Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

Insert

ERAP1 (ERAP1 Human)

Tags

myc

Mutation

G346 changed to D346

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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ERAP1-rs27895(C)

Plasmid

#206142

Purpose

Expresses the rs27895-C allele of ERAP1, myc-tagged.

Depositor

Dennis Ko

Article

Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

Insert

ERAP1 (ERAP1 Human)

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-gp78 429-611

Plasmid

#185354

Purpose

GST fusion protein for quantification of gp78 levels

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

aa 429-611 only

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 AUP1 292-410 5E

Plasmid

#185332

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 292-410 only, with mutations R382E/Q383E/K390E…

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 AUP1 292-410 A397D

Plasmid

#185333

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 292-410 only, with mutation A379D

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRgp78

Plasmid

#185344

Purpose

Expression of soluble G2BR, amino acids 575-600 of human gp78

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 aa 574-600 (G2BR) (AMFR Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 575-600 only

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRAUP1

Plasmid

#185343

Purpose

Expression of soluble G2BR, amino acids 378-404 of human AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 aa 378-404 (G2BR) (AUP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 378-404 only

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST-AUP1 379-410:Ube2G2

Plasmid

#185335

Purpose

Used for co-expression of the AUP1 G2BR with UBE2G2 for crystallography

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 aa 379-410, Ube2G2 (AUP1 Mouse, Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA10-mVenus

Plasmid

#185648

Purpose

NDUFA10 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA10 (NDUFA10 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFB6-mVenus

Plasmid

#185652

Purpose

NDUFB6 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFB6 (NDUFB6 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFS3-mVenus

Plasmid

#185649

Purpose

NDUFS3 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFS3 (NDUFS3 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA2-mVenus

Plasmid

#185645

Purpose

NDUFA2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA2 (NDUFA2 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-NDUFA10-FKBP-HA

Plasmid

#185810

Purpose

NDUFA10 subunit of Complex I fused with C-terminal FKBP and HA epitope

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA10 (NDUFA10 Human)

Use

Lentiviral

Tags

FKBP and HA

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2TKN-bp1

Plasmid

#133428

Purpose

Human BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

SREBF1 (SREBF1 Human)

Expression

Bacterial

Mutation

SREBP1a amino acids 1-50 fused to GST

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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