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pMSCV-FKBP-TRIF(trunc 1-123)

Plasmid

#221252

Purpose

Expression of TRIF truncation 6 (124-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 6 (124-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-TRIF(trunc 1-143)

Plasmid

#221253

Purpose

Expression of TRIF truncation 7 (144-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 7 (144-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-TRIF(trunc 1-163)

Plasmid

#221254

Purpose

Expression of TRIF truncation 8 (164-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 8 (164-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-TRIF(trunc 1-43)

Plasmid

#221248

Purpose

Expression of TRIF truncation 2 (44-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 2 (44-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-TRIF(trunc 1-63)

Plasmid

#221249

Purpose

Expression of TRIF truncation 3 (64-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 3 (64-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-TRIF(trunc 1-83)

Plasmid

#221250

Purpose

Expression of TRIF truncation 4 (84-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 4 (84-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-TRIF(trunc 1-103)

Plasmid

#221251

Purpose

Expression of TRIF truncation 5 (104-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 5 (104-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-TRIF(pLxIS) S209A

Plasmid

#221245

Purpose

Expression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF pLxIS domain (1-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

S209A

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-TRIF(pLxIS) L193A

Plasmid

#221246

Purpose

Expression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF pLxIS domain (1-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

L193A

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-TRIF(trunc 1-23)

Plasmid

#221247

Purpose

Expression of TRIF truncation 1 (24-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 1 (24-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-uTEV1-MAVS-mTA2

Plasmid

#171000

Purpose

HiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[R537A, R538A]

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

MAVS(tmd)[R537A, R538A]

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-uTEV1-MAVS-mTA5

Plasmid

#171003

Purpose

HiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIVLI, R537A]

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-uTEV1-MAVS-mTA6

Plasmid

#171004

Purpose

HiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-SPA mimic WT

Plasmid

#92350

Purpose

To mimic SPA RNA processing and expression

Depositor

Ling-Ling Chen

Article

Wu et al Mol Cell. 2016 Nov 3;64(3):534-548. doi:

Insert

SNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1 (SNRPN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)

Plasmid

#61935

Purpose

Expression of hRPN13 (285-407) (Q337A/Q338A) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hRPN13 (285-407) (ADRM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Construct starts at residue 285, residues Q337 an…

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS

Plasmid

#61940

Purpose

Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Inserts

hUCH37 (ISF1) (UCHL5 Human)
hNFRKB (1-156) GSGS (NFRKB Human)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Construct ends at residue 156, residues 97-100 (N…

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

Plasmid

#61941

Purpose

Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Inserts

hUCH37 (ISF1) (UCHL5 Human)
hNFRKB (1-117) (NFRKB Human)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Construct ends at residue 117, stop codon introdu…

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-MEK5 CBM-E

Plasmid

#53169

Purpose

Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

L280A/H281A/M323A/Q332A

Promoter

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-Puro-ARHGAP26

Plasmid

#69467

Purpose

Expresses ARHGAP26

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

ARHGAP26 (ARHGAP26 Human)

Use

Retroviral

Tags

HA TAG

Expression

Mutation

Promoter

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Puro- MLL3-PRKAG2

Plasmid

#69473

Purpose

Expresses MLL3-PRKAG2 fusion gene

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

MLL3-PRKAG2 (PRKAG2 Human)

Use

Retroviral

Tags

HA TAG:

Expression

Mutation

Promoter

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge