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pclbw-mitoTagRFP

Plasmid

#58425

Purpose

Mammalian expression of tagRFP targeted to the mitochondrial matrix

Depositor

David Chan

Publication

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mito-tagRFP

Use

Retroviral

Expression

Mammalian

Promoter

cmv-bactin

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pKG215

Plasmid

#45110

Depositor

Iain Cheeseman

Publication

Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP-Laci_NLS

Tags

GFP, s peptide, and tev

Expression

Mammalian

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-CHRNA7-mGFP

Plasmid

#62629

Purpose

mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Publication

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hAlpha7 (CHRNA7 Human)

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-SpyTag002-MBP

Plasmid

#102831

Purpose

Bacterial expression of SpyTag002 fused to maltose binding protein

Depositor

Mark Howarth

Publication

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyTag002-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to the N-terminus of MBP via a …

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pX330a dCas9-KRAB

Plasmid

#92361

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-KRAB

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBS500 EF1alpha-GFPcre

Plasmid

#11920

Depositor

Brian Sauer

Publication

Gagneten et al Nucleic Acids Res. 1997 Aug 15. 25(16):3

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pRK5-myc-Miro1 T18N

Plasmid

#47890

Purpose

Expresses myc tagged Miro1 T18N dominant negative mutant

Depositor

Pontus Aspenström

Publication

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 T18N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
hRIP3 GFP D160N

Plasmid

#41386

Depositor

Francis Chan

Publication

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D160N)

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMK782 Septin6-GFP

Plasmid

#38296

Depositor

Matthew Krummel

Publication

Gilden et al J Cell Biol. 2012 Jan 9;196(1):103-14.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Septin 6 (Septin6 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

22 amino acid linker sequence (added by PCR)

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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FKBP-mCherry

Plasmid

#108122

Purpose

Recruitable fluorophore

Depositor

Gerry Hammond

Publication

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)

Expression

Mammalian

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-hKCNE1

Plasmid

#53050

Purpose

Expresses beta subunit for IKs channel in mammalian cells

Depositor

Michael Sanguinetti

Publication

Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEG BacMam N term His8 eGFP 3C

Plasmid

#160684

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 eGFP 3C

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL CAGpNFlag BAF155 ires GFP

Plasmid

#24561

Depositor

Jerry Crabtree

Publication

Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

Insert

BAF155 (Smarcc1 Mouse)

Use

Lentiviral

Tags

Flag

Available since

July 16, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
PCMV-intron myc Rab2 S20N

Plasmid

#46780

Purpose

expression of S20N Rab2a in mammalian cells

Depositor

Terry Hébert

Publication

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAB2 (RAB2A Human)

Tags

myc

Expression

Mammalian

Mutation

S20N dominant negative

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pRSET his-eGFP

Plasmid

#113551

Purpose

For bacterial expression and purification of eGFP

Depositor

Jeanne Stachowiak

Publication

Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

Insert

his-eGFP

Expression

Bacterial

Mutation

A206K

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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SspBmicro-mCh-p60

Plasmid

#190168

Purpose

Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

Depositor

Anna Akhmanova

Publication

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SspBmicro-mCherry-p60

Tags

SspBmicro-mCherry

Expression

Mammalian

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTY-EF1a-wild type mKdm2b-Flag

Plasmid

#61739

Purpose

Express wild-type mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Publication

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDONR223-DDR1

Plasmid

#23910

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DDR1 (DDR1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOG231

Plasmid

#17736

Depositor

Geoff Wahl

Publication

O'Gorman et al Proc Natl Acad Sci U S A. 1997 Dec 23;94

Insert

NLS-Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
PB-gRNA-Puro

Plasmid

#121121

Purpose

Expression of a gRNA together with a Puromycin resistance

Depositor

Pablo Navarro

Publication

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA

Use

CRISPR

Expression

Mammalian

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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