Addgene

We narrowed to 21,593 results for: ESC;

Showing: 7181 - 7200 of 21593 results

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pKK233-hCALML3

Plasmid

#47599

Depositor

Emanuel Strehler

Article

Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

Insert

CALML3 (CALML3 Human)

Use

Tags

Expression

Bacterial

Mutation

contains 538 bp fragment

Promoter

trc

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pNH052

Plasmid

#42158

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

YFP-RT-YFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH054

Plasmid

#42160

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1-RT-mTFP1

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH039

Plasmid

#42151

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

CFP

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH058

Plasmid

#42161

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

TAP Tag::mTFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pKB234.1

Plasmid

#170022

Purpose

Encodes LacI-controlled expression of surface-displayed hBD3 alpha-helix fragment and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
hBD3 alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

Availability

Academic Institutions and Nonprofits only

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pKB235.4

Plasmid

#170026

Purpose

Encodes LacI-controlled expression of surface-displayed CCL20 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CCL20 Δ alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

Availability

Academic Institutions and Nonprofits only

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pKB236

Plasmid

#170027

Purpose

Encodes LacI-controlled expression of surface-displayed TCP and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
TCP

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

Availability

Academic Institutions and Nonprofits only

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pKB236.2

Plasmid

#170029

Purpose

Encodes LacI-controlled expression of surface-displayed TCP C-terminal alpha-helix fragment and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
TCP C-terminal alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

Availability

Academic Institutions and Nonprofits only

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pBAD-Flp

Plasmid

#122969

Purpose

Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

Depositor

Lydia Freddolino

Article

Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

Insert

Flp recombinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Aug. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-G-Flamp2

Plasmid

#192782

Purpose

green biosensor for cAMP in mammalian cells

Depositor

Jun Chu

Article

Liu et al Front Pharmacol. 2022 May 12;13:902290.

Insert

G-Flamp2

Use

Tags

His tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-dWPRE

Plasmid

#60907

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pmScarlet-H_C1

Plasmid

#85043

Purpose

Mammalian expression of mScarlet-H

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

mScarlet-H

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-GFP-aequorin

Plasmid

#101011

Purpose

Construct for expression of a calcium sensitive luciferase reporter, GFP-aequorin, and hygromycin resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

Expression

Mutation

Promoter

Ribi

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMito-LSSmOrange

Plasmid

#37135

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_Long_G_31AACTdel

Plasmid

#237350

Purpose

Expression plasmid for RSV Long G

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV Long G from Genbank Accesion AY911262.1

Use

Tags

Expression

Mammalian

Mutation

31 amino acid cytoplasmic tail deletion

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_Long_F

Plasmid

#237349

Purpose

Expression plasmid for RSV Long F

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV Long F from Genbank Accesion AY911262.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B1_F

Plasmid

#237353

Purpose

Expression plasmid for RSV B1 F

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV B1 F human codon optimized from Genbank Accesion AF013254

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mTurquoise2

Plasmid

#98886

Purpose

Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

Plasmid

#175540

Purpose

Expression of HaloTag9 in the endoplasmid reticulum of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CalR-HaloTag9-KDEL

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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