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pCytERM-miRFP2-N1

Plasmid

#197173

Purpose

Expresses the fusion protein of CytERM-miRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-miRFP2

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Aq(2nd)

Plasmid

#183089

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Pxyl-ftsZ-sfGFP

Plasmid

#174505

Purpose

Integrated plasmid harboring ftsZ-sfGFP gene, inducible by xylose (sequence of sfGFP optimized)

Depositor

Suliana Manley

Article

Mahecic et al Nat Methods. 2022 Sep 8. pii: 10.1038/s4

Insert

ftsZ

Tags

superfold GFP

Expression

Bacterial

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMT335-mScarletI

Plasmid

#174506

Purpose

High-copy number plasmid (pMT335) harboring the mScarletI gene for its cytoplasmic expression, inducible by vanillate (sequence of mScarletI optimized)

Depositor

Suliana Manley

Article

Mahecic et al Nat Methods. 2022 Sep 8. pii: 10.1038/s4

Insert

mScarletI

Tags

mScarletI

Expression

Bacterial

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Violet

Plasmid

#183139

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Violet

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Violet

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-TalO8 (Blank-CoTrIP)

Plasmid

#178303

Purpose

Blank CoTrIP plasmid

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Type

Empty backbone

Tags

NA

Expression

Yeast

Promoter

NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-hmKO2

Plasmid

#182733

Purpose

Transient expression of humanized mKO2 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

hmKO2

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-hKO1

Plasmid

#182731

Purpose

Transient expression of humanized KO1 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

hKO1

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-mKO1

Plasmid

#182723

Purpose

Transient expression of mKO1 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

mKO1

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-mKO2

Plasmid

#182724

Purpose

Transient expression of mKO2 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

mKO2

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-KO1

Plasmid

#182722

Purpose

Transient expression of KO1 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KO1

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-hmKO1

Plasmid

#182732

Purpose

Transient expression of humanized mKO1 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

hmKO1

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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BiDi-[mp-mEGFP]mp-mCherry2

Plasmid

#182870

Purpose

Bi-directional expression vector of monomers with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

Insert

myr-palm-mEGFP, myr-palm-mCherry2

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP

Plasmid

#182853

Purpose

Hetero-trimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mEYFP-mCherry2-mEGFP

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0U6K8E5

Plasmid

#163266

Purpose

Inducible expression of UniProt identifier A0A0U6K8E5

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0U6K8E5

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0U5XJG3

Plasmid

#163238

Purpose

Inducible expression of UniProt identifier A0A0U5XJG3

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0U5XJG3

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJRK252

Plasmid

#173749

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Pelt-2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR

Expression

Worm

Promoter

Pelt-2

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJRK251

Plasmid

#173748

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Pwrt-2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR

Expression

Worm

Promoter

Pwrt-2

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJRK137

Plasmid

#173745

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::mKate2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pKP932

Plasmid

#167652

Purpose

Express colicinB-His6, M261C

Depositor

Kathleen Postle

Article

Skare et al J Bacteriol. 1989 Aug;171(8):4442-7. doi

Insert

cba-his6 M261C

Expression

Bacterial

Mutation

cba-his6 with M261C mutation

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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