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We narrowed to 12,187 results for: LEA

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Showing: 7201 - 7220 of 12187 results

  3. pcDNA3.1-Ctdnep1_D67E-2xFlag

    Plasmid
    #196519
    Purpose
    Expression of Ctdnep1_D67E-2xFlag
    Depositor
    Edgar Gomes
    Publication
    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ctdnep1_D67E-2xFlag (CTDNEP1 Human)
    Tags
    2xFlag
    Expression
    Mammalian
    Mutation
    D67E mutation (phosphastase dead)
    Promoter
    CMV promoter; T7 promoter
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    196519-plasmid-map-sequence-id-386702

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  7. pcDNA3-EGFP-AC8(1-106)

    Plasmid
    #181958
    Purpose
    Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.
    Depositor
    Jin Zhang
    Publication
    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-AC8(1-106) (Adcy8 Rat)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181958-plasmid-map-sequence-id-363198

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  11. pCEP4-myc-S (B.1.351 lineage / South African variant)

    Plasmid
    #169849
    Purpose
    Mammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / South African variant) with N-terminal c-myc tag
    Depositor
    Erik Procko
    Publication
    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Protein S
    Tags
    HA leader and c-myc tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    169849-plasmid-map-sequence-id-329351

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  12. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  13. H2B-mScarlet- hRec8(297-506)-hMeikin(1-332, E151R, R154E)- mNeonGreen

    Plasmid
    #174716
    Purpose
    Stable expression of fluorescent Separase cleavage sensor with human Rec8 fused to human Meikin
    Depositor
    Iain Cheeseman
    Publication
    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8
    Insert
    Rec8 (REC8 Human)
    Use
    Retroviral
    Tags
    H2B-mScarlet and mNeonGreen
    Expression
    Mammalian
    Mutation
    hRec8: 297-506; hMeikin: 1-332, E151R, R154E
    Available since
    Oct. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    174716-plasmid-map-sequence-id-343358

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  15. pCI-Flag-NEDD4L∆WW1,2,4

    Plasmid
    #171137
    Purpose
    expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells
    Depositor
    Wesley Sundquist
    Publication
    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NEDD4L (NEDD4L Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed tryptophan 221 to alanine, tryptophan 453…
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171137-plasmid-map-sequence-id-338242

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  18. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AMPKsub-YPet-Kras
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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  19. t1-405(del37/GAG)

    Plasmid
    #166129
    Purpose
    Expression of human talin-1 head (aa1-405) in E. coli. Contains 37 amino acid deletion in F1-loop, replaced with Gly-Ala-Gly. N-terminal His6, Xpress-epitopeDLYDDDDK) and enterokinase cleavage site.
    Depositor
    Vesa Hytönen
    Publication
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(del37/GAG) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    aa1-405, with 37 aa deletion in the F1-loop which…
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166129-plasmid-map-sequence-id-330258

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  20. pcDNA-GFP-EphrinB3-Flag(A227Y)

    Plasmid
    #155013
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1
    Depositor
    Sinisa Urban
    Publication
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EphrinB3 (EPHB3 Human)
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    A227Y
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155013-plasmid-map-sequence-id-310418

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Showing: 7201 - 7220 of 12187 results