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pGEL053

Plasmid

#137907

Purpose

OsBiP1 promoter driven Cas9-csy4 system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1752

Plasmid

#139877

Purpose

Expression plasmid for naturally occurring 5xMCE protein (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

Nitrincola_nitratireducens_D791_00999

Expression

Bacterial

Mutation

Nitrincola_nitratireducens_D791_00999(47-644)-6xH…

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pKM45

Plasmid

#154152

Purpose

Firefly luciferase with no promoter for negative control for luciferase assays

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pIntegrase-AntiDsRed

Plasmid

#139677

Purpose

Cassette for testing phiC31 Integrase activity using a DsRed2 fluorescent reporter

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed Integrase

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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Scarlet I-FRET 10-Cerulean3

Plasmid

#139374

Purpose

FRET Standard

Depositor

Richard Day

Article

Day et al PLoS One. 2021 Dec 23;16(12):e0261660. d

Insert

Cerulean3 N1

Expression

Mammalian

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-iRISB

Plasmid

#138979

Purpose

Light-controlled tri-directional targeting of endogenous proteins

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL050

Plasmid

#137904

Purpose

Dual-mini35S driven Cas9-Csy4 system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL051

Plasmid

#137905

Purpose

Dual-mini35S-enhancer driven Cas9-Csy4 system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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UL91-2xStrep pCDNA4

Plasmid

#120375

Purpose

Expresses HCMV UL91with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL91 (UL91 Human Cytomegalovirus (HHV-5))

Tags

2xStrep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAc-Cdc3-Cdc10-SNAP

Plasmid

#131161

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

Cdc3 + Cdc10-SNAP

Use

Bicistronic

Tags

Cdc10: SNAP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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IgH intron enhancer

Plasmid

#127247

Purpose

To maximize gene expression in B cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

heavy chain enhancer

Expression

Mammalian

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-amo

Plasmid

#113190

Purpose

Expresses amo in vertebrate cells

Depositor

Craig Montell

Article

Watnick et al Curr Biol. 2003 Dec 16;13(24):2179-84.

Insert

amo (Pkd2 Fly)

Use

In vitro transcription

Expression

Mammalian

Mutation

Y602H

Promoter

simian CMV IE94

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pNB2629

Plasmid

#113394

Purpose

MET25p-MS2-RFP-CYC1 TT

Depositor

Suzanne Sandmeyer

Article

Clemens et al Virus Res. 2013 Feb;171(2):319-31. doi:

Insert

MS2 bacteriophage coat protein

Tags

red fluorescent protein (RFP), codon-optimized fo…

Expression

Yeast

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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MEI-S332-GFP genomic in pCaSpeR

Plasmid

#113174

Purpose

In P element vector for transposon insertion into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo)

Use

P insertion vector

Tags

GFP

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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KG#244

Plasmid

#110927

Purpose

Vector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cord

Depositor

Kenneth Miller

Article

Edwards et al J Cell Biol. 2009 Sep 21;186(6):881-95.

Inserts

unc-129 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pKSJ375

Plasmid

#103064

Purpose

Expresses ImmE1, immunity to colicin E1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Insert

colicin E1 immunity protein (pColE1_26, imm E. coli)

Expression

Bacterial

Promoter

E1 immunity protein promoter from ColE1

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pTC102

Plasmid

#91176

Purpose

a derivative of pDIRECT_37J with translation initiation sequence optimized for dicots

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAWK

Plasmid

#62713

Purpose

Donor vector for amiR-eGFP knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419/FLPe

Use

RNAi

Expression

Mammalian

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pARD (pKD-G4)

Plasmid

#62683

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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