We narrowed to 18,937 results for: Tec

1. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

   Plasmid

   #149426

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Inserts

   U6.3-gRNAs[Sxl, bTub]
   Hsp70Bb-Cas9-T2A-eGFP-p10
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pv6_dPC_Chromo_TAF3_Phd

   Plasmid

   #179403

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_Phd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   dPC_Chromo - TAF3_Phd
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1352wUV2 F2bbs kan GCANNNAGGk

   Plasmid

   #18752

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   3 tandem zinc fingers
   

   Expression

   Bacterial

   Mutation

   A Kan cassette (1090bp) is inserted between two b…

   Available Since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV5-Flag-RapR-p38

   Plasmid

   #25935

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

   Insert

   RapR-p38 (Mapk14 Mouse, Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGS1022

   Plasmid

   #59576

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
   

   Use

   E. coli cloning vector

   Tags

   Twin-Strep tag

   Promoter

   None

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. mWasabi-Mito-7

   Plasmid

   #56508

   Purpose

   Localization: Mitochondria, Excitation: 493, Emission: 509

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Mito (COX8A Human)
   

   Tags

   mWasabi

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-RIPK3[S199A][S227A]-FLAG

   Plasmid

   #199323

   Purpose

   double mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited)

   Depositor

   Adrian Press

   Article

   Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

   Insert

   Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 199 and 227 to Alanine

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB3039(XII-2 MarkerFree)

   Plasmid

   #73279

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUPD2 HDV (GB1444)

   Plasmid

   #160576

   Purpose

   Hepatitis Delta Virus ribozyme signal for the assembly of gRNA expression cassettes.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   HDV
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXSIP-GPR35-FLAG-APEX

    Plasmid

    #204706

    Purpose

    expresses GPR35-FLAG-APEX in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    G protein-coupled receptor 35 (GPR35 Human)
    

    Tags

    FLAG, APEX

    Expression

    Mammalian

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-TPI_PTC160-xrRNA-4H

    Plasmid

    #108369

    Purpose

    Expresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Expression

    Mammalian

    Mutation

    PTC160

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p1.2-Zeo-eGFP

    Plasmid

    #162743

    Purpose

    Fluorescent reporter for CHO cells expression studies

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Insert

    enhanced green fluorescent protein
    

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 PA-mCit

    Plasmid

    #113443

    Purpose

    ProteinA-mCitrine control expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ269A2

    Plasmid

    #164714

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI
    

    Use

    CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ265

    Plasmid

    #164712

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    rAPOBEC1-zCas9(D10A)-UGI
    

    Use

    CRISPR; Gateway compatible rapobec1-cbe_v01 entry…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Myc-Foxo1-WT 6KR

    Plasmid

    #17558

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 6KR (Foxo1 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K242R, K246R, K259R, K262R, K271R, K291R

    Promoter

    CMV

    Available Since

    Oct. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

17. 1352wUV2 F3bbs kan NNNTGGGCGk

    Plasmid

    #18756

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-dCpf1-BE0

    Plasmid

    #107678

    Purpose

    Expresses dLbCpf1-BE0 in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE0
    

    Expression

    Mammalian

    Mutation

    D832A/E925/D1148A

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCellFree_G03 FOXM

    Plasmid

    #67118

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FOXM (FOXM1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pPSP6+t-gfp

    Plasmid

    #48130

    Purpose

    Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    gfp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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